Objective(s) This study aimed to investigate and to compare the effects of nifedipine and amlodipine, dihydropyridine (DHP) calcium channel blockers (CCBs) on perfusion pressure of isolated perfused rat kidney. Materials and Methods Following the establishment of renal perfusion with a constant baseline pressure of 85-95 mmHg, the renal vasculature was constricted by phenylephrine (PE) injec...

The objective of this study was to isolate hepatocytes of the proximal half (Zones 1 and 2) or distal half (Zones 2 and 3) of the liver acinus. The zonal origin of the isolated hepatocytes was recognized by: the presence in hepatocytes of a fluorescent marker, acridine orange, selectively delivered to either the proximal or the distal half of the acinus by in situ perfusion prior to cell isolat...

PURPOSE Human hepatocellular carcinoma (HCC) has unique vascular features, which require selective imaging of hepatic arterial perfusion and portal venous perfusion with vascular catheterization for sufficient evaluation. Unlike in humans, vessels in mice are too small to catheterize, and the importance of separately imaging the feeding vessels of tumors is frequently overlooked in hepatic tumo...

objective(s) this study aimed to investigate and to compare the effects of nifedipine and amlodipine, dihydropyridine (dhp) calcium channel blockers (ccbs) on perfusion pressure of isolated perfused rat kidney. materials and methods following the establishment of renal perfusion with a constant baseline pressure of 85-95 mmhg, the renal vasculature was constricted by phenylephrine (pe) injectio...

PURPOSE Liver metastases are the sole or life-limiting component of disease in the majority of patients with ocular melanoma who recur. Because median survival after diagnosis of liver metastases is short and no satisfactory treatment options exist, we have conducted clinical trials evaluating isolated hepatic perfusion (IHP) for patients afflicted with this condition. EXPERIMENTAL DESIGN Twe...

sterone or [16B-”H]betamethasone was given at a dose of 0.23pmol/kg body wt. by intraperitoneal or intravenous route, and bile samples were collected at 30min intervals. In some experiments the animals were pretreated with daily doses (100mg/kg body wt., intraperitoneally) of phenobarbital for 3 consecutive days. In the isolated-liver perfusion experiments tissue-culture medium 199 (100ml) cont...

Abstract Introduction Normothermic machine perfusion (NMP) is a method of organ preservation that aims to replicate the physiological environment, achieved by perfusing livers with blood-based perfusate at inflow pressures and temperature. NMP also permits viability assessment through evaluation flow rates portal vein hepatic artery. In addition this, biochemical gas analysis can be performed p...

Patients diagnosed with primary or metastatic liver cancer face a daunting future that is complicated by limited treatment options. Percutaneous hepatic perfusion is a novel approach to chemotherapy delivery that offers significant benefits over contemporary modalities. Percutaneous hepatic perfusion is a procedure in which a chemotherapeutic agent is administered at high doses via the hepatic ...