Background and Aims: Mesenchymal stem cells (MSCs) are a preferred cell source for the generation of hepatocyte-like cells in regenerative medicine. They can be isolated from different sources, including adipose tissues. The Raman spectroscopy approach was evaluated for quick and efficient identification of MSCs differentiation status and a broader perspective on cell differentiation. Material...

Vascular and lymphatic malformations represent a challenge for clinicians. The identification of inherited somatic mutations in important signaling pathways, including the PI3K (phosphoinositide 3-kinase)/AKT (protein kinase B)/mTOR (mammalian target rapamycin), RAS (rat sarcoma)/RAF (rapidly accelerated fibrosarcoma)/MEK (mitogen-activated protein kinase)/ERK (extracellular signal-regulated ki...

Experimental rat models and clinical trials in human patients with liver cirrhosis show evidence that supplementation hepatotropic factors provides therapeutic benefits. This form of support has not yet been described dogs multiple acquired portosystemic shunts (MAPSS) despite similarities between both pathological conditions. Especially hepatocyte growth factor (HGF) branched chain amino acids...

Background: Human FIX (hFIX) gene transfer into hepatocytes has provided a novel approach for treatment of hemophilia B. To obtain an improved expression of hFIX, the functional hFIX-expressing plasmids with appropriate intron-derived fragments which facilitate transcription and promote an efficient 3′-end formation of mRNAs are required.Objectives: We ai...

background: stearic acid is known as a potent anti-inflammatory lipid. this fatty acid has profound and diverse effects on liver metabolism. the aim of this study was to investigate the effect of stearic acid on markers of hepatocyte transplantation in rats with acetaminophen (apap)-induced liver damage. methods: wistar rats were randomly assigned to 10-day treatment. stearic acid was administe...

GOALS To evaluate the therapeutic efficacy of rat bone marrow mesenchymal stem cells (BMSCs) induced into hepatocyte-like cells and of un-induced BMSCs in acute liver failure rats. METHODS BMSCs in highly homogenous passage 3 were cultured using the whole bone marrow adherent culture method. Hepatic-related characters were confirmed with morphology, RT-PCR analysis, glycogen staining and albu...

After surgical removal of two-thirds of the liver, remaining hepatocytes replicate and restore hepatic mass within 2 weeks. This process must be initiated by signals extrinsic to the hepatocyte, but it remains unclear whether subsequent events leading to DNA synthesis (S phase) are regulated by circulating or locally produced growth factors (a noncell autonomous response), or by a program intri...

The formation of hepatic bile requires that water be transported across liver epithelia. Rat hepatocytes express three aquaporins (AQPs): AQP8, AQP9, and AQP0. Recognizing that cholesterol and sphingolipids are thought to promote the assembly of proteins into specialized membrane microdomains, we hypothesized that canalicular bile secretion involves the trafficking of vesicles to and from local...

Hepatic failure (HF) is caused by several factors, which induce liver cell damage and dysfunction. However, the specific mechanism of HF remains to be fully elucidated. The present study aimed to investigate the underlying cause of hepatocyte injury and liver dysfunction. Liver cells were isolated from healthy female Sprague‑Dawley rats, aged between 6 and 8 weeks, weighing ~230 g. The liver ce...

The cellular content of vitamin E was measured in isolated rat hepatocytes exposed to various types of chemical injury. Vitamin E was determined as alpha-tocopherol by HPLC with in-line uv and electrochemical detection. The cytotoxicity of diquat, a redox cycling compound, was accompanied by a decrease in cellular alpha-tocopherol and a stimulation of lipid peroxidation. Both the loss of alpha-...