BACKGROUND The pathogenesis of polycystic kidney disease (PKD) remains unclear despite the identification of the genes responsible for hereditary PKD. In this study, we investigated the alteration of gene expressions in an acquired PKD model induced by 2-amino-4,5-diphenylthiazole (DPT) using the differential display method. METHODS Kidney mRNA from a Sprague-Dawley rat fed with 1% DPT for 4 ...

A full-length cDNA encoding rat type 2C (IA) protein phosphatase was isolated from a kidney cDNA library. The cDNA was identified by screening the library with oligonucleotides based on a partial amino acid sequence determined from purified rat liver phosphatase. This clone is 2.35 kilobase pairs long and has a single extended translation reading frame that predicts a 382-amino acid protein of ...

We previously reported the isolation of a cDNA encoding the liver-specific isozyme of rat S-adenosylmethionine synthetase from a lambda gt11 rat liver cDNA library. Using this cDNA as a probe, we have isolated and sequenced cDNA clones for the rat kidney S-adenosylmethionine synthetase (extrahepatic isoenzyme) from a lambda gt11 rat kidney cDNA library. The complete coding sequence of this enzy...

Objective(s):MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression. They have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in various forms of chronic kidney disease (CKD). In the present study, we elucidated the role of microR...

the objective of this study was to determine the chronology of changes in blood pressure in renalhypertension induced by solid plexiglass clips in rats. saw blades with the thickness of 0.21-0.22 mm wereused to make clips sized 4 × 2 × 2 mm from a piece of 2-mm thick plexiglass. rats were subjected to shamoperation or left renal arterial clipping, and 1, 2, 3 and 4 weeks later blood pressure, a...

introduction: ischemia/reperfusion (ir) injury involves a complex interrelated sequence of events. high levels of nitric oxide (no) are generated with inducible form of nitric oxide synthase (inos) leading to the renal ir injury and glutathione (gsh) depletion. the present study was designed to investigate the effect of l-nil (n6- (1-iminoethyl)-l- lysine.hydrochloride), a selective inhibitor o...

  Urinary N-acetyl-β-D-glucosalninidase (NAG) has been proved to be a useful marker of early renal injury as a result of factors such as lead toxicity. In this study the effect of lead acetate on the kidney and its correlation with the nitric oxide (NO) system was investigated by determining the NAG release in perfused kidney of rat. Lead acetate caused a time- and dose-dependent increase in en...

In the present study, we investigated the therapeutic potential of mesenchymal stem cells (MSCs) in a rat acute renal failure (ARF) model and explored the possible in vivo and in vitro mechanisms of action. Rat and human MSCs were isolated from bone marrow. After being co-cultured with injured kidney tissues in trans-well dishes in vitro, the rat MSCs became rounded renal tubular epithelial-lik...

We have tested rat liver fructose-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 3.1.3.11) and three other gluconeogenic fructose-bisphosphatases as substrates for the catalytic subunit of cyclic AMP-dependent protein kinase. In contrast to the rat liver enzyme, homogeneous preparations of mouse liver, rabbit liver, and pig kidney fructose-bisphosphatase could not be phospho...

Controversial results regarding the role of vasopressin as a mediator of ethanol-induced diuresis raise the question, whether ethanol may stimulate diuresis directly on the level of the kidney, possibly by its vasodilating properties. We thus studied the effects of different ethanol-concentrations (0.5 ‰, 1 ‰, 2 ‰, and 4 ‰; n = 4 to 6, respectively) – in the presence of 10 mU/l vasopressin in t...