Boiled homogenates of kidneys from volume-expanded and hydropenic rats were subjected to column chromatography. The fraction eluting within the range of partition coefficients (Kav) 0.76-0.89 (fraction III) was lyophilized and the effects of this semipurified preparation were assessed on short-circuit current (SCC) across isolated frog skin, on rat kidney cortex Na-K-ATPase activity, and on sod...

Abstract The consumption of inadequately thermally treated fish is a public health risk due to the possible propagation Anisakis larvae. present study demonstrated physiological and histopathological changes that accompanied an oral inoculation crude extracts from fresh Type II (L3) in rats. Worms were isolated marine examined identified using light scanning electron microscopy. was performed 6...

The transport of L-proline was studied in brush-border membrane vesicles isolated from the kidneys of newborn rats. In contrast with the rapid initial uptake with an 'overshoot' observed in adult vesicles, uptake by the newborn vesicle was slow, showed no 'overshoot', and proline continued to accumulate at a time when the adult vesicle had already equilibrated. L-Proline transport in the newbor...

1. The interaction of unconjugated bilirubin with peritubular cell membranes of the rat kidney was studied by means of an isolated rat-kidney preparation applying the multiple-indicator-dilution technique. 2. Inulin was used as an extracellular marker and p-aminohippuric acid as a model of organic anion that interacts with the peritubular membrane. 3. A single renal artery injection of a mixtur...

1. The effect of D-aldosterone and dexamethasone on kallikrein release was studied in the isolated rat kidney perfused with modified Krebs-Henseleit buffer. 2. Animals received D-aldosterone or dexamethasone (10 microgram/100 g body weight) daily for 3 days. Their isolated kidneys were then perfused with the same steroids at rates of 2.0-2.5 microgram/min for 30 min. 3. Dexamethasone increased ...

OBJECTIVE To study the renal excretion and kidney accumulation of pentamidine, a potentially nephrotoxic compound, in the isolated perfused rat kidney (IPK). MATERIALS AND METHODS IPK experiments (3-4 per treatment group) were conducted using male Sprague-Dawley rats (250-350 g). Dose proportionality studies were carried out over a pentamidine dosing range of 80-4000 microg, designed to targe...

Nephrotoxicity is an important limitation to the clinical use of colistin against Pseudomonas aeruginosa and other gram-negative pathogens. Previous work reported net tubular reabsorption of colistin by the kidney in vivo, but there is no knowledge of its disposition within the kidney. This study investigated the renal disposition and potential transport mechanisms of colistin in the isolated p...

Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 microg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate...

1. A technique for perfusing the isolated rat kidney is described. It is primarily designed for the study of renal metabolism but is also suitable for studying some aspects of the secretory function; this was normal with respect to minimal glucosuria. The glomerular filtration rate as measured by creatinine clearance was lower than in vivo and slowly decreased with time. 2. Gluconeogenesis from...

Suspensions of freshly isolated rat kidney cells were used to study glutathione biosynthesis and utilization for amino acid translocation. Incubation of cells isolated from diethylmaleate-perfused kidneys in the presence of glutamate, glycine, and cystine led to replenishment of cellular glutathione and to accumulation of glutathione disulfides in the medium. Cystine could be replaced by cystei...