Isolated rat hepatocytes were used to determine the cytotoxicity of peripheral blood mononuclear cells (PBM) from patients with alcoholic liver disease (ALD) and chronic active hepatitis (CAH). The specificity of the cytotoxic effect on liver cells was monitored using rat kidney cells. PBM from patients with CAH and ALD showed increased spontaneous cell-mediated cytolysis (SCMC) for hepatocytes...

Enzymatic dispersion and density gradient (Percoll) sedimentation were used to isolate a population of renin-containing, granule-laden cells (density 1.067 g/ml) from rat kidney cortex. Using immunohistochemistry (light microscopy) and electron microscopy, we defined the presence and ability of these cells to store renin protein(s). A 1000 base pair rat renin complementary DNA was used to show ...

Isolated brush border membranes from rat kidney bind 13H]phlorizin specifically. A “high affinity” binding site (Ka (the association constant) = 5.0 X lo6 1 X mole-r at 150 mu sodium, pH 7.4,37”) is enriched in the brush border membrane and is not present in kidney mitochondria or red blood cell ghosts of the rat. The concentration of the high affinity receptors in the membrane fraction is 40 p...

To begin elucidating the biochemical basis of the polarized membrane features of the blood-brain barrier (BBB), a series of immunochemical and immunoperoxidase studies were initiated with bovine brain microvessels that make up the BBB in vivo. A rabbit antiserum was prepared against isolated bovine brain BBB plasma membranes. The bovine microvessel plasma membranes were radioiodinated with chlo...

introduction: there have been many studies in recent years on the role of nitric oxide (no) in acute renal failure. in this study, the effects of inhibition or interoduction of no synthase on renal toxicity of gentamicin were investegated. materials & methods: isolated kidneys of male rats were perfused with tyrode buffer (t) as the control group, t+l-argrinine as group 2, t+l-name as group 3, ...

Recently, we isolated the multispecific organic anion transporter (OAT1) from the rat kidney, which plays important roles in the renal elimination of endogenous and exogenous organic anions including clinically important drugs. In the present study, we cloned and characterized human OAT1. Two cDNA clones, hOAT1-1 cDNA and hOAT1-2 cDNA, were isolated from a human kidney cDNA library, whose amino...

In the rat isolated perfused kidney, arachidonic acid elicits cyclooxygenase-dependent vasoconstriction through activation of PGH2/TxA2 receptors; responses are enhanced in kidneys from diabetic rats. This study examined the roles of cyclooxygenase-1/cyclooxygenase-2 in the enhanced renal vasoconstrictor effect of arachidonic acid in streptozotocin-diabetic rats. Release of 20-HETE was also det...

In the rat isolated perfused kidney, arachidonic acid elicits cyclooxygenase-dependent vasoconstriction through activation of PGH2/TxA2 receptors; responses are enhanced in kidneys from diabetic rats. This study examined the roles of cyclooxygenase-1/cyclooxygenase-2 in the enhanced renal vasoconstrictor effect of arachidonic acid in streptozotocin-diabetic rats. Release of 20-HETE was also det...

BACKGROUND In vitro, cold-induced injury is an important contributor to renal tubular cell damage. It is mediated by iron-dependent formation of reactive oxygen species and can be prevented by iron chelation. We studied whether iron chelators can prevent cold-induced damage in the isolated perfused rat kidney (IPK) model both after cold perfusion (CP) and after cold storage (CS). We hypothesize...

Hisashi Ozasa MD, Kidney Center, Tokyo Women’s Medical College, Kawada-cho 8-1, Shinjuku-ku, Tokyo 162 (Japan) Dear Sir, Methylguanidine (MG) is a suspected ure-mic toxin that accumulates in renal failure [1]. Aoyagi et al. [2] reported that MG was produced from creatinine (Cr) in the presence of active oxygen in an experimental system using isolated hepatocytes. However, Yoko-zawa et al. [3] h...