A new isozyme of cytochrome P-450, designated from 3a on the basis of its relative electrophoretic mobility, has been purified to homogeneity from liver microsomes of rabbits treated chronically with ethanol. This cytochrome has the highest activity of the known rabbit P-450 isozymes in the oxidation of ethanol to acetaldehyde. In view of the reports of others that the hepatotoxicity of acetami...

The electrophoretic patterns of esterase (E-l), alcohol dehydrogenase (ADH), and glutamate oxaloacetate transaminase (GOT) isozymes were studied in two Spanish populations of the lily Scilla autumnalis with Bchromosome carrying individuals. The E-l isozyme activity appears only in those individuals with B-chromosomes. None of the bulbs free of B’s show it. Five bulbs, mosaic for B-content, were...

Esterase activity between the resistant Crawford and susceptible CSMA strains of German cockroach, Blattella germanica (L.), was compared with the substrates alpha- and beta-naphthyl acetate across sex and nymphal age classes. Esterase isozyme analysis with native polyacrylamide gel electrophoresis also was conducted to identify quantitative and qualitative differences between strains, sexes, a...

Agents such as prostaglandins E1 and I2 which elevate cAMP levels in platelets also increase cAMP phosphodiesterase activity. Since much of the cAMP phosphodiesterase activity in human platelets is due to the cGMP-inhibited isozyme (Macphee, C. H., Harrison, S. A., and Beavo, J. A. (1986) Proc. Natl. Acad. Sci. U. S. A. 83, 6600-6663), we examined the regulation of this isozyme by prostaglandin...

Six cathepsin D isozymes have been purified from porcine spleen using a large scale purification procedure. Five isozymes, I to V, have an identical molecular weight of 50,000 and are similar in specific activity. Isozymes I to IV contained two polypeptide chains each. The light and heavy chains have Mr = 15,000 and 35,000, respectively. Isozyme V is a single polypeptide. The molecular weight o...

Received April 12,1978 SUMMARY Isoproterenol injection produces cardiac hypertrophy and salivary gland enlargement in rats. After subcutaneous injection (5 mg/kg) twice daily for 10 days, the soluble extracts from these and other tissues were subjected to DEAE-cellulose chromatography and the activity of isozymes I and II of cyclic AMP-dependent protein kinase was measured. The activity of isoz...

Glycogen synthase, the rate-limiting enzyme in glycogen biosynthesis, has been postulated to exist as isozymes in rabbit liver and muscle (Camici, M., Ahmad, Z., DePaoli-Roach, A. A., and Roach, P. J. (1984) J. Biol. Chem. 259, 2466-2473). Both isozymes share a number of properties including multiple phosphorylation of the enzyme subunit. In the present study, we determined the amino acid seque...

Ca2#/calmodulin-dependent protein kinase II (CaMKII) isozyme variability is the result of alternative usage of variable domain sequences. Isozyme expression is cell type-specific to transduce the appropriate Ca2# signals. We have determined the subcellular targeting domain of "E CaMK-II, an isozyme that induces neurite outgrowth, and of a structurally similar isozyme, !C CaMK-II, which does not...

A novel matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry method has been developed to quantitate cytochrome P450 (P450) isozymes based on their unique isozyme-specific tryptic peptides. It was shown that the molar ratio of P450 isozyme-specific peptides is linearly proportional to the mass peak area ratio of corresponding peptides not only in simple two-p...

The Fusarium wilt caused by Fusarium oxyspoum f. sp. cubense (Foc) is a major biotic constraint for banana production. The characteristics of F. oxyspoum f. sp. cubense isolates were investigated using electrophoretic studies of isozyme and whole-cell protein. The morphological characteristics of the isolates were very similar to each other. All the Foc isolates were pathogenic to banana cultiv...