Previous studies have shown that cardiac inward rectifier potassium current (I(K1)) channels are heteromers of distinct Kir2 subunits and suggested that species- and tissue-dependent expression of these subunits may underlie variability of I(K1). In this study, we investigated the contribution of the slowly activating Kir2.3 subunit and free intracellular polyamines (PAs) to variability of I(K1...

CrkRS (Cdc2-related kinase, Arg/Ser), or cyclin-dependent kinase 12 (CKD12), is a serine/threonine kinase believed to coordinate transcription and RNA splicing. While CDK12/CrkRS complexes were known to phosphorylate the C-terminal domain (CTD) of RNA polymerase II (RNA Pol II), the cyclin regulating this activity was not known. Using immunoprecipitation and mass spectrometry, we identified a 6...

Escherichia coli is a major cause of enteric/diarrheal diseases, urinary tract infections, and sepsis. E. coli K1 is the leading gram-negative organism causing neonatal meningitis, but the microbial basis of E. coli K1 meningitis is incompletely understood. Here we employed comparative genomic hybridization to investigate 11 strains of E. coli K1 isolated from the cerebrospinal fluid (CSF) of p...

Sickling-induced cation fluxes contribute to cellular dehydration of sickle red blood cells (SS RBCs), which in turn potentiates sickling. This study examined the inhibition by dipyridamole of the sicklinginduced fluxes of Na1, K1, and Ca11 in vitro. At 2% hematocrit, 10 mM dipyridamole inhibited 65% of the increase in net fluxes of Na1 and K1 produced by deoxygenation of SS RBCs. Sickle-induce...

Abstract We investigate the relation between the cone Cn of n×n copositive matrices and the approximating cone K1 n introduced by Parrilo. While these cones are known to be equal for n ≤ 4, we show that for n ≥ 5 they are unequal. This result is based on the fact that K1 n is not invariant under diagonal scaling. We show in fact that for any copositive matrix which is not the sum of a nonnegati...

BACKGROUND Klebsiella pneumoniae causing community-acquired pyogenic liver abscess complicated with metastatic meningitis and endophthalmitis has emerged recently, most frequently associated with the K1 capsular type. METHODS A bacteriophage (NTUH-K2044-K1-1) that infects K. pneumoniae NTUH-K2044 (capsular type K1) was isolated and characterized. RESULTS The phage infected all K1 strains, a...

The K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that pu...

Vitamin K denotes a group of molecules that are needed for the synthesis of certain proteins, mostly required for blood coagulation. Five isoforms of vitamin K exists: vitamin K1, K2, K3, K4, and K5. Vitamin K1 (phytonadione, CAS number 84-800/81818-54-4) has been widely used in cosmetics as it is claimed to reduce the purpura and hyperpigmentation associated with surgical and laser therapies (...

A gene library of genomic DNA Klebsiella aerogenes of capsular serotype K1 was constructed in E. coli LE392 using the cosmid pMMB33. Culture filtrates of E. coli recombinants were screened by ELISA for extracellular polysaccharides specific for K. aerogenes K1. Extracellular polysaccharide extracts from K. aerogenes K1 and 3% of the E. coli recombinants contained immunoprotective extracellular ...