OBJECTIVE Patients who appear to have both stimulating and blocking TSHR autoantibodies in their sera have been described, but the two activities have not been separated and analysed. We now describe the isolation and detailed characterization of a blocking type TSHR monoclonal autoantibody and a stimulating type TSHR monoclonal autoantibody from a single sample of peripheral blood lymphocytes....

A (δ ≥ k1, δ ≥ k2)-partition of a graph G is a vertex-partition (V1, V2) of G satisfying that δ(G[Vi]) ≥ ki for i = 1, 2. We determine, for all positive integers k1, k2, the complexity of deciding whether a given graph has a (δ ≥ k1, δ ≥ k2)-partition. We also address the problem of finding a function g(k1, k2) such that the (δ ≥ k1, δ ≥ k2)-partition problem is NP-complete for the class of gra...

Keratins 1 (K1) and 10 (K10) are the predominant cytoskeletal intermediate filaments of epidermal cells during transition from the proliferative to the terminal differentiation stage. In situ, formation of the K1/K10 intermediate filament network occurs in the cytoplasm of cells with a preexisting cytoskeleton composed of keratins 5 and 14. To define cytoskeletal interactions permissive for for...

A newly introduced compound, EPC-K1, represents a phosphate diester linkage of vitamin E and vitamin C. The effect of EPC-K1 on the reperfusion injury was evaluated in a heterotopic cardiac transplantation model using syngenic combination rats. Prior to the warm ischemia, 12mg EPC-K1/kg was administered intravenously to donor rats. After 15 min of warm ischemic time, hearts were harvested and p...

Two classes of phages yield profoundly different levels of recovery in mice experimentally infected with an Escherichia coli O18:K1:H7 strain. Phages requiring the K1 capsule for infection (K1-dep) rescue virtually all infected mice, whereas phages not requiring the capsule (K1-ind) rescue modest numbers (∼30%). To rescue infected mice, K1-ind phages require at least a 10(6)-fold-higher inoculu...

Background In the past two decades, Vitamin K has been receiving more attention due to its role in bone health and metabolism. The bone mineral density does not remain steady with age, particularly declining after menopause. Objective This study is aimed to investigate the relationship between bone mineral density and serum vitamin K1 levels in post-menopausal women, and to evaluate serum vit...

Juel, C., H. Pilegaard, J. J. Nielsen, and J. Bangsbo. Interstitial K1 in human skeletal muscle during and after dynamic graded exercise determined by microdialysis. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 278: R400– R406, 2000.—Interstitial K1 concentrations were measured during one-legged knee-extensor exercise by use of microdialysis with probes inserted in the vastus lateralis...

Cougnon, Marc, Patrice Bouyer, Frédéric Jaisser, Aleksander Edelman, and Gabrielle Planelles. Ammonium transport by the colonic H1-K1-ATPase expressed in Xenopus oocytes. Am. J. Physiol. 277 (Cell Physiol. 46): C280–C287, 1999.—Functional expression of the rat colonic H1-K1-ATPase was obtained by coexpressing its catalytic a-subunit and the b1-subunit of the Na1-K1-ATPase in Xenopus laevis oocy...

Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.