A monoclonal antibody against rat liver glutathione S-transferase B, which consists of subunits Ya (Mr 27,000) and Yc (Mr 29,500), was produced in a mouse hybridoma system. The monoclonal antibody, BE6Yc1, was of the immunoglobulin G1 class and specifically recognized glutathione S-transferase B showing no cross-reactivity against the dimeric form of Ya, ligandin, in enzyme-linked immunosorbent...

to assess toxicity in control and selenium-deficient rats given paraquat and diquat. Lipid peroxidation was measured by determining ethane production rates of intact animals; toxicity was assessed by survival and by histological and serum enzyme evidence of liver and kidney necrosis. Paraquat and diquat were both much more toxic to selenium-deficient rats than to control rats. Diquat (19.5 umol...

BACKGROUND This study examined the effect of grape seed proanthocyanidin extract (GSPE) on lipid peroxidation content and activity of tissue antioxidant enzymes, including catalase, superoxide dismutase and glutathione peroxidase in diabetic rats. METHODS Thirty male rats were divided into three groups of 10 rats each: control, diabetic and diabetic groups that received 500 mg/kg GSPE for 6 w...

Introduction. Dysfunction of the kidneys after a bone fracture significantly increases recovery time patients, risk complications and disability. Recently, mesenchymal stem cells have been used to restore both kidney tissue. Our study was devoted effect on processes lipid peroxidation (LPO) work antioxidant system injury.Materials methods. The carried out 120 male white rats weighing from 190 2...

The present study evaluated the adverse effects of three widely used insecticides in Egyptian environment on mitochondrial bioenergetic and oxidative stress biomarkers rat kidney. Chlorpyrifos, cypermethrin, imidacloprid were orally administrated to male albino rats at 1/50 LD50 for 28 days by 5 doses /week. caused a significant vivo decrease activities biomarkers; NADH dehydrogenase, adenosine...

Effects of L-arginine (L-arg) and aminoguanidine (AG) on the nephrotoxicity induced by cyclosporine (CsA) were investigated. After injection of CsA (15 mg kg(-1) day (-1)i.p. for 10 days), it induced nephrotoxicity, manifested biochemically by a significant elevation of serum urea and creatinine. In addition, a marked increase in lipid peroxides measured as malondialdehyde (MDA) as well as a si...

The present work studied the protective effect of Opuntia ficus indica f. inermis fruit juice (OFIJ) against ethanol-induced renal dysfunction in rats. Chronic ethanol administration (3 g/kg b.w) during 12 weeks to Wistar rats, significantly (p < 0.01) increased the serum and kidney malondialdehyde (MDA), the serum contents of urea, creatinine, gamma glutamyl transferase (GGT) and the urinary g...

The effects of Arabic gum (AG) against nephrotoxicity of mercury (Hg), an oxidative-stress inducing substance, in rats were investigated. A single dose of mercuric chloride (5 mg/kg intraperitoneal injection) induced renal toxicity, manifested biochemically by a significant increase in serum creatinine, blood urea nitrogen, thiobarbituric acid reactive substances, and total nitrate/nitrite prod...

Organotin compounds are a versatile group of organometallic chemicals that are used in a variety of industrial and agricultural applications. Tributyltin (TBT), a common organotin, brings about severe spermatotoxic and organotoxic effects. However, information about the adverse effects of TBT on liver, kidney and testis is scanty. Hence, the present study was undertaken to elucidate the TBT-med...

The present study was designed to investigate the effect of Spirulina platensis (SP) supplemented diets on growth performance, digestive enzymes, hepatic antioxidants and innate immunity biomarkers in juvenile grass carp (Ctenopharyngodon idella). Two hundred forty juveniles (average weight 4.81 ± 0.13 g) were divided into four treatment groups triplicates (20 fish/replicate) fed with containin...