We used the RAPD-PCR method for distinguishing the main Old World Leishmania parasites Leishmania tropica, L. major, and L. infantum and applied it to Sauroleishmania species from Iran. Twelve out of 21 tested primers were suitable for identification of these parasites. The Jaccard similarity index was 0.30 for L. tropica and L. infantum as well as for L. major and L. infantum. The similarity c...

Introduction: Depuis 1995, le Maroc a connu une réactivation des foyers de leishmanioses cutanées (LC) à L. major et une nouvelle répartition géographique des foyers à L. tropica. Le but de cette étude est de comparer les aspects épidémio-cliniques associés aux LC potentiellement dûes à L. major et à L. tropica. Méthodes: Une étude rétrospective a colligé 268 cas de LC au service de dermatologi...

17β-estradiol (E2), the predominant and most biologically active form of estrogen has been shown to modulate various macrophage activities, sometimes with opposite effects, related to experimental conditions. The aim of this study was to investigate the effect of E2 alone or in combination with PKA inhibitor H-89 or recombinant human chorionic gonadotropin (rhCG) on leishmanicidal activity and ...

BACKGROUND Cutaneous leishmaniasis (CL) caused by Leishmania tropica is endemic in Kerman, southeastern Iran. While dogs have long been implicated as the main domestic reservoirs of L. infantum, etiological agent of zoonotic visceral leishmaniasis (ZVL), they can also carry L. tropica infection. The objective of the present study was to determine molecular identity and to evaluate histopatholog...

Three species of L. tropica, L. major and L. infantum are known as main causal agents of leishmaniasis have been reported in Iran. Since cutaneous leishmaniasis (CL) is endemic in North East of Iran, in the present work, 50 Leishmania positive isolates from human cases in Mashhad (Center of Razavi province, North East of Iran), were genotyped by means of polymerase chain reaction-restriction f...

We report experimental infection and transmission of Leishmania tropica (Wright), by the blood-feeding sand fly Phlebotomus duboscqi (Neveu-Lemaire). Groups of laboratory-reared female sand flies that fed "naturally" on L. tropica-infected hamsters, or artificially, via membrane feeding device, on a suspension of L. tropica amastigotes, were dissected at progressive time points post-feeding. Ac...

BACKGROUND Viscerotropic leishmaniasis caused by Leishmania tropica poses a significant problem in the diagnosis and treatment management. Since differential gene expression is more important in outcome of the infection, we employed proteomic approach to identify potential proteins involved in visceralization of L. tropica. METHODS The proteomes profiling of L. tropica isolated from cutaneous...

The possibility that purine inhibitors or analogs might be effective antileishmanial agents led to the determination of the antileishmanial activity of mycophenolic acid and allopurinol in vitro. The drugs were tested against Leishmania tropica amastigotes (mammalian forms) within human macrophages, a model in which achievable serum concentrations of antileishmanial agents currently in use elim...

BACKGROUND Limited data are available regarding topical and systemic therapies for Leishmania tropica in children. OBJECTIVE We sought to characterize the clinical presentation and evaluate the efficacy and safety of topical and systemic treatments in pediatric patients infected with L tropica. METHODS A retrospective study was performed on 47 children with L tropica cutaneous leishmaniasis...

Phlebotomus sergenti is a natural vector of Leishmania tropica. However, the ability of P. sergenti to transmit L. tropica by bite has not been proven experimentally yet. We have transmitted L. tropica to golden hamsters and BALB/c mice by the bite of P. sergenti. Sand flies and Leishmania both originated from an anthroponotic cutaneous leishmaniasis focus in Urfa, Turkey. P. sergenti females f...