نتایج جستجو برای: lactate
تعداد نتایج: 30620 فیلتر نتایج به سال:
We sought to determine (a) if early lactate clearance is associated with improved survival in emergency department patients with severe sepsis and (b) the concordance between central venous oxygen saturation (ScvO2) optimization and lactate clearance during early sepsis resuscitation. Within a multicenter shock research network that uses quantitative resuscitation for severe sepsis, we analyzed...
Abnormal electrophoretic pattern of serum lactate dehydrogenase isoenzyme is shown. In this case electrophoretic pattern showed a sharp single band between lactate dehydrogenase 4 and lactate dehydrogenase 5 and broad band between lactate dehydrogenase 2 and lactate dehydrogenase 4. This abnormality was suggested to be caused by the presence of lactate dehydrogenase and immunoglobulin G (kappa ...
The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentratio...
Activity of D-lactate dehydrogenase (D-LDH) was shown not only in cell extracts from Megasphaera elsdenii grown on DL-lactate, but also in cell extracts from glucose-grown cells, although glucose-grown cells contained approximately half as much D-LDH as DL-lactate-grown cells. This indicates that the D-LDH of M. elsdenii is a constitutive enzyme. However, lactate racemase (LR) activity was pres...
To evaluate the potential role of mitochondrial lactate dehydrogenase (LDH) in tissue lactate clearance and oxidation in vivo, isolated rat liver, cardiac, and skeletal muscle mitochondria were incubated with lactate, pyruvate, glutamate, and succinate. As well, alpha-cyano-4-hydroxycinnamate (CINN), a known monocarboxylate transport inhibitor, and oxamate, a known LDH inhibitor were used. Mito...
To study the role of muscle mass and muscle activity on lactate and energy kinetics during exercise, whole body and limb lactate, glucose, and fatty acid fluxes were determined in six elite cross-country skiers during roller-skiing for 40 min with the diagonal stride (Continuous Arm + Leg) followed by 10 min of double poling and diagonal stride at 72-76% maximal O(2) uptake. A high lactate appe...
Mechanisms of hepatic lactate uptake were studied in the gulf toadfish, Opsanus beta by following the accumulation of [C]lactate by isolated hepatocytes in vitro. Uptake rates were linear for up to 5 s at external lactate concentrations of 1 and 20mmoll~'. 5-s uptake rates for several concentrations in the physiological range (i.e. 0-2-20mmoll") yielded a linear double reciprocal plot (l/v vs l...
Lactic acid is produced as an end product of glycolysis in rainbow trout white muscle following exhaustive exercise. The metabolically produced lactic acid causes an intramuscular acidosis that must be cleared, either via net transport out of the muscle or by conversion to glycogen, thereby replenishing the muscle energy store. Trout muscle has been shown to retain lactate and utilise it as a s...
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