1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...

1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...

BACKGROUND The LH-induced folliculo-luteal transformation is connected with alterations of the gene expression profile in cells of the granulosa layer. It has been described that hypoxic conditions occur during luteinization, thus favoring the formation of L-lactate within the follicle. Despite being a product of anaerobic respiration, L-lactate has been shown to act as a signaling molecule aff...

The production of glycolytic end products, such as lactate, usually evokes a cellular shift from aerobic to anaerobic ATP generation and O2 insufficiency. In the classical view, muscle lactate must be exported to the liver for clearance. However, lactate also forms under well-oxygenated conditions, and this has led investigators to postulate lactate shuttling from non-oxidative to oxidative mus...

Many factors impact the fluctuating rates of glucose flux and clearance in one’s daily life (meal size and composition, gastric emptying rate, physical activity, etc.). With this constantly changing need for circulating insulin abundance, it is challenging for the treatment strategy to be entirely successful in individuals with type 1 diabetes (T1D), and transient periods of hypoglycemia occur....

Classic neuroenergetic research has emphasized the role of glucose, its transport and its metabolism in sustaining normal neural function leading to the textbook statement that it is the necessary and sole metabolic fuel of the mammalian brain. New evidence, including the Astrocyte-to-Neuron Lactate Shuttle hypothesis, suggests that the brain can use other metabolic substrates. To further study...

The effect of a single bout of exhaustive exercise on muscle lactate transport capacity was studied in rat skeletal muscle sarcolemmal (SL) vesicles. Rats were assigned to a control (C) group (n = 14) or an acutely exercised (E) group (n = 20). Exercise consisted of treadmill running (25 m/min, 10% grade) to exhaustion. SL vesicles purified from C and E rats were sealed because of sensitivity t...