The high infectivity, difficulty to diagnose, and drug resistance of melioidosis limited the timeliness treatment. Lateral flow assay (LFA) was operated in this research provide an instant diagnosis method, a fast antibody rapid matching test strategy based on LFA developed select most sensitive specific pair antibodies. Compared traditional ELISA new limits pairing time 3 h without any complex...

To improve the detection sensitivity of neomycin (NEO) in milk, we produced a sensitive monoclonal antibody (mAb) against NEO and developed lateral flow immunoassay based on amorphous carbon nanoparticles (ACNPs-LFA). First, conjugated to carrier protein prepare mAbs. We obtained six mAbs: mAb 1C6, 1D3, 2D3, 4D5, 5D1, 5H1. characterised mAbs by indirect competitive enzyme-linked immunosorbent a...

Evaluating the clinical performance of available methods to detect antibodies against Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has become a primordial issue in laboratories. The aim this study was evaluate two for SARS-CoV-2 detection, an automated Chemiluminescent Immunoassay (CLIA) and immunochromatographic Lateral-Flow Assay (LFA) patients with positive reverse transcript...

AIM The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized buffalo farm. MATERIALS AND METHODS A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay (iELISA), and compet...

BACKGROUND Cryptococcosis is a common opportunistic infection of human immunodeficiency virus (HIV)-infected individuals mostly occurring in resource-limited countries. This study compares the performance of a recently developed lateral flow immunoassay (LFA) to blood culture and enzyme immunoassay (EIA) for the diagnosis of cryptococcosis. METHODS Archived sera from 704 HIV-infected patients...

The paper-based immunoassay for point-of-care diagnostics is widely used due to its low cost and portability over traditional lab-based assays. Lateral-flow immunoassay (LFA) is the most well-established paper-based assay since it is rapid and easy to use. However, the disadvantage of LFA is its lack of sensitivity in some cases where a large sample volume is required, limiting its use as a dia...

Wearable lateral flow assay (LFA) devices in the point-of-care (POC) diagnosis are an emerging technology due to their ease of use, affordability, and robustness. Recent advances wearable allow rapid self-diagnosis using colorimetry. Visual readouts LFA strips often lead inaccurate readout different lighting conditions. Smartphone-based increasingly popular among POC platforms. Myriad challenge...

A highly rapid, one-step immunoassay of high sensitivity C-reactive protein (hsCRP) using a biosensor with a vertical flow immunoassay (VFA) was developed. The VFA biosensor was primarily composed of a sample pad, conjugate pad, FTH film and nitrocellulose (NC) membrane, which were all vertically stacked upon one another. Anti-hsCRP and secondary antibodies were consecutively immobilized on the...

The lateral-flow immunoassay (LFA) is an inexpensive point-of-care (POC) paper-based diagnostic device with the potential to rapidly detect disease biomarkers in resource-poor settings. Although LFA is inexpensive, easy to use, and requires no laboratory equipment, it is limited by its sensitivity, which remains inferior to that of gold standard laboratory-based assays. Our group is the only on...

We compared the performance of four assays for the detection of cryptococcal antigen in serum samples (n = 634) and cerebrospinal fluid (CSF) samples (n = 51). Compared to latex agglutination, the sensitivity and specificity of the Premier enzyme immunoassay (EIA), Alpha CrAg EIA, and CrAg lateral flow assay (LFA) were 55.6 and 100%, 100 and 99.7%, and 100 and 99.8%, respectively, from serum sa...