The increased production of Reactive Oxygen Species (ROS) in plant leaf tissues is a hallmark of a plant’s reaction to various environmental stresses. This paper describes an automatic segmentation method for scanned images of cucurbits leaves stained to visualise ROS accumulation sites featured by specific colour hues and intensities. The leaves placed separately in the scanner view field on a...

Nitrogen deficiency severely inhibits leaf growth. This response was analysed at the cellular level by growing Lolium perenne L. under 7.5 mM (high) or 1 mM (low) nitrate supply, and performing a kinematic analysis to assess the effect of nitrogen status on cell proliferation and cell growth in the leaf blade epidermis. Low nitrogen supply reduced leaf elongation rate (LER) by 43% through a sim...

Understanding leaf and fruit abscission is essential in order to develop strategies for controlling the process in fruit crops. Mechanisms involved in signalling leaf and fruit abscission upon induction by abscission agents were investigated in Citrus sinensis cv. ‘Valencia’. Previous studies have suggested a role for phospholipid signalling; hence, two phospholipase D cDNA sequences, CsPLDa1 a...

Rice spotted leaf 6 (spl6) mutant produces lesions caused by spontaneous cell death in the absence of pathogenic infection. Expression of this genetic trait was developmentally programmed. After the tillering stage, small red and brown lesions were initiated in groups on the leaf blade. Eventually, the lesions formed parallel lines along the midrib of the leaf. Under light and transmission elec...

Cellulose synthase-like (CSL) genes are predicted to catalyse the biosynthesis of non-cellulosic polysaccharides such as the β-D-glycan backbone of hemicelluloses and are classified into nine subfamilies (CSLA-CSLH and CSLJ). The CSLD subfamily is conserved in all land plants, and among the nine CSL subfamilies, it shows the highest sequence similarity to the cellulose synthase genes, suggestin...

The maize mutant narrow sheath (ns) displays a leaf shape and plant stature phenotype that suggests the preprimordial deletion of a leaf domain. The ns mutant phenotype is inherited as a recessive, duplicate-factor trait, conditioned upon homozygosity for each of the two unlinked mutations narrow sheath-1 (ns1) and narrow sheath-2 (ns2). Mutant leaves are missing a large domain including the le...

Sugar beet plants (Beta vulgaris L. var. F5855441) were germinated and cultured under standardized environmental conditions for 28 days. Potassium deficiency was then induced by withholding K from the culture solution. Changes in CO(2) and water vapor exchange rates and surface temperatures of individual attached leaves were measured with time after K cut-off, along with changes in the concentr...

Leaves experience predictable 24-h (diel) fluctuations in environmental factors such as light and temperature, as well as in energy availability. Accordingly, leaf growth is among the many processes regulated by the circadian clock. Despite the importance of leaf growth to the plant, it is relatively poorly understood, in large part due to the difficulty of tracking growth in leaves compared wi...

Foliar trichomes of gray-leaved Tillandsioideae (Bromeliaceae) are highly reflective, suggesting a role in protecting the leaf against direct sunlight in exposed niches. The performance of photosystem II, as denoted by the chlorophyll fluorescence characteristic Fv /Fm , was determined for seven Tillandsia species and Vriesea barclayana that were exposed to excessive light, with trichomes eithe...