Leaf colour is usually used as a guide for assessments of nutrient status and plant health. We propose a new inexpensive, hand-held and easy-to-use technique for the detection of chlorophyll content and foliar nitrogen content in plants based on leaf colour. This method provides a rapid analysis and data storage at minimal cost and does not require any technical or laboratory skills. Most of th...

Assessing nitrogen (N) status is important from economic and environmental standpoints. To date, many spectral indices to estimate cotton chlorophyll or N content have been purely developed using statistical analysis approach where they are often subject to site-specific problems. This study describes and tests a novel method of utilizing physical characteristics of N-fertilized cotton and comb...

Mesophyll protoplasts and bundle sheath strands of maize (Zea mays L.) leaves have been isolated by enzymatic digestion with cellulase. Mesophyll protoplasts, enzymatically released from maize leaf segments, were further purified by use of a polyethylene glycol-dextran liquid-liquid two phase system. Bundle sheath strands released from the leaf segments were isolated using filtration techniques...

ABSTRACT. Net photosynthesis, dark respiration, chlorophyll and carbohydrate content, and leaf and shoot growth in plants of evergreen olive (Olea europaea L.) grown under controlled conditions were measured to assess changes in carbon balance during leaf development of the 6th, 12th, and 16th node (from the base, first flush) through expansion to maturity. Shoot and leaves expanded in a sigmoi...

In situ optical meters are widely used to estimate leaf chlorophyll concentration, but non-uniform chlorophyll distribution causes the opticalmeasurement to vary widely among species for the same chlorophyllconcentration. Over 30 studies have sought to quantify the in situ/in vitro (optical/absolute) relationship, but neither chlorophyll extraction normeasurement techniques for ...

A central reaction of chlorophyll breakdown, porphyrin ring opening of pheophorbide a to the primary fluorescent chlorophyll catabolite (pFCC), requires pheophorbide a oxygenase (PAO) and red chlorophyll catabolite reductase (RCCR), with red chlorophyll catabolite (RCC) as a presumably PAO-bound intermediate. In subsequent steps, pFCC is converted to different fluorescent chlorophyll catabolite...

Chlorosis level is a useful parameter to assess imidazolinone resistance in sunflower (Helianthus annuus L.). The aim of this study was to quantify chlorosis through two different methods in sunflower plantlets treated with imazapyr. The genotypes used in this study were two inbred lines reported to be different in their resistance to imidazolinones. Chlorosis was evaluated by spectrophotometri...

Leaf coloration is one of the most important and attractive characteristics of Ananas comosus var. bracteatus. The chimeric character is not stable during the in vitro tissue culturing. Many regenerated plants lost economic values for the loss of the chimeric character of leaves. In order to reveal the molecular mechanisms involved in the albino phenotype of the leaf cells, the physiological an...

Radiative transfer models have seldom been applied for studying heterogeneous grassland canopies. Here, the potential of radiative transfer modeling to predict LAI and leaf and canopy chlorophyll contents in a heterogeneous Mediterranean grassland is investigated. The widely used PROSAIL model was inverted with canopy spectral reflectance measurements by means of a look-up table (LUT). Canopy s...

Plant phenology, a sensitive indicator of climate change, influences vegetation-atmosphere interactions by changing the carbon and water cycles from local to global scales. Camera-based phenological observations of the color changes of the vegetation canopy throughout the growing season have becomepopular in recent years. However, the linkages between camera phenological metrics and leaf bioche...