Membrane fractions from seedlings of four soybean [Glycine max (L.) Merr.] lines were examined by radioimmunoassay and hemagglutination assay for the 120,000 dalton soybean lectin. Two of the lines (Sooty and T-102) are genotypically lele and lack buffer-soluble soybean lectin; the remaining two lines (Beeson and Harosoy 63) are Le and produce seeds that contain the lectin (Su et al. 1980 Bioch...

Many fungi are known to secrete lectins, but their functional roles are not clearly understood. Sclerotium rolfsii, a soilborne plant pathogenic fungus capable of forming fruiting bodies called sclerotial bodies, secrete a cell wall-associated Thomsen-Friedenreich antigen-specific lectin. To understand the functional role of this lectin, we examined its occurrence and expression during developm...

An endogenous beta-galactoside-specific lectin has previously been isolated from rabbit bone marrow. The quantification of extracted lectin now indicates that approximately 75% of the lectin is intracellular in marrow. Indirect immunofluorescence studies show the extracellular lectin is associated with the erythroblast cell surface and is also found in some acellular areas of the marrow stroma....

Gold nanoparticles and gold nanoshells functionalised with a selection of carbohydrates: glyco-nanoparticles towards lectin detection in dipstick assay.

The isolation of lectins by affinity chromatography with crosslinked hemicelluloses has been a common practice because the variety glycosides that they present, improving different kinds lectins, such as galactose ligands.Lectins for carbohydrates is so specific simple configuration chiral carbon can affect affinity, and there are more related to alfa-galactosidic than beta-galactosidic residue...

The purpose of this article is to report that some anti-A1 react at 37°C are IgG antibodies and clinically significant, as they can cause the destruction a proportion A1 cells in vivo following transfusion red blood cells. Therefore, when serum an individual contains anti-A1, further testing plasma against group A1, A2, O by referencing lectin (Dolichos biflorus lectin) must be performed. Confi...

The tissue concentration of an endogenous beta-galactoside-specific lectin is developmentally regulated in rabbit tissues during foetal and neonatal development. Immunoassay data and localization studies both indicate that the lectin is particularly associated with late embryonic and early postnatal development. No lectin could be detected in embryos prior to 21 days of gestation, whereas many ...

Changes in lectin activity during development of embryonic chick skin were studied. In the dorsal skin of the chick embryo in which feathers were formed, lectin activity first increased, during the period of dermal condensation, and then it decreased during the development of feathers. A similar change in lectin activity was also found in the anterior shank skin, the prospective scale region of...

it is well known that glycoconjugate components of the cell surface and extracellular matrix, play an essential role(s) in many developmental phenomena such as cell differentiation, migration, and cellular interactions. the purpose of this study was to investigate distribution of this macromolecules during differentiation of the notochord and paraxial mesoderm. formalin fixed paraffin sections ...

objective(s):the aim of this study was to evaluate distribution and changes of glycoconjugates of retinal photoreceptors during both pre- and postnatal development. materials and methods: tissue sections from days 15 to 20 of wistar rat embryos and 1 to 12 postnatal days of rat newborns including developing eye were prepared for lectinhistochemistry technique. horseradish peroxidase (hrp)-label...