Two sibling foxhounds born to a Leishmania seropositive bitch were presented after testing seropositive for Leishmania. Leishmania infantum infection was detected via histopathology, culture, and quantitative polymerase chain reaction (q-PCR). This is the first report of natural infection with Leishmania infantum with the possibility for vertical transmission in North America.

A cryptic sexual reproductive cycle in Leishmania has been inferred through population genetic studies revealing the presence of hybrid genotypes natural isolates, with attempts made to decipher strategies by studying complex chromosomal inheritance patterns. more informative approach is study products controlled, laboratory-based experiments where known strains or species are crossed sand fly ...

the subcutaneous inoculation of 16 white mice and 10 golden hamsters with l.tropica major resulted in the development of leishmanial lesions in all, becoming generalized in some.two of 10 hamsters inoculated intraperitoneally with this parasite developed generalized lesions. on the other hand, the inoculation of l. tropica minor to 128 mice subcutaneously and 170 mice intraperitoneally did not ...

Leishmaniasis is an infectious disease caused by Leishmania species. Leishmania amazonensis is a New World Leishmania species belonging to the Mexicana complex, which is able to cause all types of leishmaniasis infections. The L. amazonensis reference strain MHOM/BR/1973/M2269 was sequenced identifying 8,802 codifying sequences (CDS), most of them of hypothetical function. Comparative analysis ...

INTRODUCTION During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. METHODS Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. RESULTS PCR assays for Leishmania subgenus identifica...

Transgenic Leishmania major and Leishmania donovani axenic promastigotes constitutively expressing mCherry were used for in vitro antileishmanial drug screening. This method requires minimal sample manipulation and can be easily adapted to automatic drug tests, allowing primary high-throughput screenings without the need for expensive and sophisticated instruments.

Three species of L. tropica, L. major and L. infantum are known as main causal agents of leishmaniasis have been reported in Iran. Since cutaneous leishmaniasis (CL) is endemic in North East of Iran, in the present work, 50 Leishmania positive isolates from human cases in Mashhad (Center of Razavi province, North East of Iran), were genotyped by means of polymerase chain reaction-restriction f...

cutaneous leishmaniasis (cl) remains a serious public health concern in kerman province, eastern iran. this study was aimed to conduct a comprehensive review and highlights various aspects of cl in the province of kerman.this article mainly focuses on the studies published in the past 26 years on cl in the province. current data for the present status were obtained through the provincial health...

Activated natural killer (NK) cells release interferon (IFN)-γ, which is crucial for the control of intracellular pathogens such as Leishmania. In contrast to experimental murine leishmaniasis, the human NK cell response to Leishmania is still poorly characterized. Here, we investigated the interaction of human blood NK cells with promastigotes of different Leishmania species (Leishmania major,...

Background: Leishmania is a pathogenic parasite which infects mononuclear cells in vertebrate hosts. Different strategies have been taken to develop immunity against Leishmania . DCs loaded with immunogenic antigen have resulted in different levels of Th1-type immune response and cytotoxic T lymphocytes (CTL) activity. Objective: To evaluate the potency of DCs primed with soluble Leishmania mex...