Phlebotomus sergenti Parrot, 1917 is the main vector of Leishmania tropica; however, its broad geographical range and molecular heterogeneity suggest possible variability in vector competence. We infected laboratory-reared P. sergenti originating from Turkey and Israel to compare their susceptibility to L. tropica. In both tested groups, heavy late-stage infections with the presence of metacycl...

Objective Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods. Methods Novy-MacNeal-Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal...

cutaneous leishmaniasis (cl) remains a serious public health concern in kerman province, eastern iran. this study was aimed to conduct a comprehensive review and highlights various aspects of cl in the province of kerman.this article mainly focuses on the studies published in the past 26 years on cl in the province. current data for the present status were obtained through the provincial health...

materials and methods the l. tropica strain was attenuated using continuous passaging 20 times. the infectivity of l. tropica was confirmed in balb/c mice. the l. tropica resistant strain was produced in vitro using a continuous increase in drug pressure. the cross resistance of l. tropica to other drugs was also investigated. results after 20 continuous passages, the balb/c mice tested negativ...

Plasmodium falciparum Amebae (Intestinal) Babesia species Entamoeba histolytica Flagellates (Leishmaniae, Trypanosomes) Entamoeba dispar* Leishmania tropica complex Entamoeba hartmanni Leishmania mexicana complex Entamoeba coli Leishmania braziliensis complex Entamoeba polecki Leishmania donovani complex Endolimax nana Leishmania peruviana Iodamoeba bütschlii Trypanosoma brucei gambiense Blasto...

this cross-sectional study was designed to isolate of leishmania spp from cutaneous leishmaniasis patients and characterized them by rapd-pcr technique. eighty- seven leishmania isolates from 112 samples were collected from cutaneous leishmaniasis (cl) patients who referred to mashhad health centers from august 2002 to may 2004. desirable samples (87 isolates) were characterized by rapd-pcr met...

Phlebotomus sergenti is a natural vector of Leishmania tropica. However, the ability of P. sergenti to transmit L. tropica by bite has not been proven experimentally yet. We have transmitted L. tropica to golden hamsters and BALB/c mice by the bite of P. sergenti. Sand flies and Leishmania both originated from an anthroponotic cutaneous leishmaniasis focus in Urfa, Turkey. P. sergenti females f...

BACKGROUND Cutaneous leishmaniasis constitutes a major public health problem in many parts of the world including Iran. The primary objective of this study was to identify Leishmania species in endemic districts of Kerman Province, south-eastern Iran. METHODS This study was conducted by random sampling as cross- sectional descriptive between 2008 and 2010. Overall, 203 skin scraping smears we...

Antibacterial activity of CNSs against Staphylococcus aureus and Escherichia coli was estimated. Higher inhibition zone 18 mm 20 were observed S. E.coli, respectively, at a concentration 2 mg/ml carbon nanosphere after 24 hrs incubation 37 ºC. In vitro cytotoxicity experiment performed on two parasite strains Leishmania donovani tropica by using MTT assay. L. revealed more sensitiv to the than ...

the subcutaneous inoculation of 16 white mice and 10 golden hamsters with l.tropica major resulted in the development of leishmanial lesions in all, becoming generalized in some.two of 10 hamsters inoculated intraperitoneally with this parasite developed generalized lesions. on the other hand, the inoculation of l. tropica minor to 128 mice subcutaneously and 170 mice intraperitoneally did not ...