In this study, 191 culture isolates were recovered from suspected samples of animals and humans in Ellinghausen McCullough Johnson and Harris (EMJH) medium and assessed for its morphological features by dark field microscopy. Extracted DNA from individual culture was subjected to different PCR assays for identification and characterization of leptospira. Out of 99 positive leptospira cultures, ...

ABSTRACT 1 ........................................................................................................................................ 3 Review of 1999 International Conference on Alternatives to Animal Challenge Tests in the Batch Control of Leptospira Vaccines for Veterinary Use Lukas Bruckner, Institute of Virology and Immunoprophylaxis (IVI), Mittelhäusern, Switzerland1 ..........

BACKGROUND Infection with Leptospira may occur by contact with Leptospira-infected animals. Waste pickers are in contact with rodents and dogs while picking in the garbage. Whether waste pickers are at risk for Leptospira infection is largely unknown. This study was aimed to determine the association of Leptospira IgG seroprevalence with the occupation of waste picking, and to determine the epi...

BACKGROUND Leptospira can be found in soil. However, it is unclear whether occupational exposure to soil may represent a risk for Leptospira infection in humans. Therefore, we sought to determine the association of Leptospira IgG seroprevalence with the occupation of gardener, and to determine the epidemiological characteristics of gardeners associated with Leptospira exposure. METHODS We per...

PCR amplification of the nearly full-length of virulence flagellin gene (flaB) was employed for rapid identification of Leptospira spp and of Leptospira-specific 16S rDNA (rrs) for differentiation from other bacteria. This approach distinguished pathogenic from non-pathogenic Leptospira strains, and the generation of restriction fragment length polymorphism profiles using a combination of restr...

BACKGROUND. We tested the hypothesis that patients who have recovered from leptospirosis have peripheral blood memory T cells that are specific for Leptospira or Leptospira protein antigens. METHODS. Peripheral blood mononuclear cells (PBMCs) were obtained from patients who had recovered from leptospirosis, as well as from control individuals. PBMCs were assessed for in vitro proliferation, phe...

A retrospective study was conducted to identify and describe the distribution pattern of Leptospira serogroups in domestic animals in France. The population consisted of cattle herds and dogs with clinically suspected leptospirosis that were tested at the "Laboratoire des Leptospires" between 2008 and 2011. The laboratory database was queried for records of cattle and dogs in which seroreactivi...

the importance of cats in the leptospira epidemiology is due to the possibility of transferring leptospirosis to wild and domesticated animals. the purpose of this survey was to determine the prevalence of leptospira infection in shorthair cats in different location of mashhad, iran. totally, 147 blood samples were taken from 42 (28.57%), 52 (35.37%) and 53 (36.05%) households, stray and cats w...

Leptospirosis caused by spirochete bacterium of genus Leptospira affects humans and animals worldwide. Rodents are major reservoirs of leptospires whereas wetland and aquatic migratory birds also carry and transmit leptospires. Leptospirosis studies in fish are lacking in African countries despite favourable environment and abundant reservoirs, which can spread leptospires into aquatic habitats...

Southern margin of the Caspian Sea due to keeping of domestic animals and abundance of surface water reservoirs is prone to infection with Leptospira sp.. This study was performed to determine anti-Leptospira antibody frequency in Golestan province, southeast of the Caspian Sea in the north of Iran. This study was performed on 1028 people in Golestan province. Sampling was stratified. Leptospir...