Prognosis for patients suffering from T-ALL is still very poor and new strategies for T-ALL treatment are urgently needed. Our study shows potent anti-leukemic effects of the myxobacterial V-ATPase inhibitor Archazolid A. Archazolid A reduced growth and potently induced death of leukemic cell lines and human leukemic samples. By inhibiting lysosomal acidification, Archazolid A blocked activatio...

BACKGROUND Leukemia is characterized by the uncontrolled accumulation of white blood cells. Recently, cytokines have been used in immunotherapy, which is a new strategy for leukemia treatment. OBJECTIVE To investigate the effect of cytokines on induction of apoptosis in acute leukemic cell lines; HL-60, MV4-11, K-562 and Molt-4 and in addition, to study the involvement of nitric oxide (NO) in...

Proline-rich homeobox (Prh) is a novel human homeobox-containing gene recently isolated from the CD34+ cell line KG-1A, and whose expression appears mainly restricted to hematopoietic tissues. To define the pattern of Prh expression within the human hematopoietic system, we have analyzed its constitutive expression in purified cells obtained from normal hematopoietic tissues, its levels of tran...

FK866 is a specific inhibitor of NAMPT and induces apoptosis of leukemic cells by depletion of intracellular NAD(+). Since up-regulation of NAMPT is associated with several cases of cancers, including leukemias, we asked whether in leukemic cells inhibition of NAMPT involves p53 pathway. We observed that FK866 induced apoptosis and reduced cell proliferation in NB-4, OCI-AML3 and MOLM-13 cell l...

Samples of leukemic cell DNA from 14 children with acute nonlymphocytic leukemia (ANLL) and 4 human myeloid leukemia cell lines were analyzed for rearrangement in the heavy chain region of the immunoglobulin gene. The diagnosis of ANLL was confirmed in all patients by morphological, cytochemical, and immunologic studies. By restriction endonuclease digestion and hybridization with cloned heavy ...

introduction: interleukin-2 (il-2) as a t helper type 1 (th1) cytokine has an important role background and objective: interleukin-2 (il-2) as a t helper type 1 (th1) cytokine has an important role in activation, growth and differentiation of several immune cells. moreover il-2 is known as a pro-inflammatory and anti-tumoral cytokine. in addition deysregulation of il-2 in some diseases such as ...

the aim of this work was to see whether tamarin immunisation with recombinant vaccinia virus expressing epstein barr virus latent proteins could prime t cells which were, on activation, able to inhibit the outgrowth of epstein barr virus transformed cells in vitro. the vaccination appeared to be successful as all vaccinated tamarins developed vaccinia lesions. however, the vaccination protocol ...

The apoptogenic activity of Litchi chinensis leaf extract (LCLE) was investigated against three human leukemic cell lines-U937, K562 and HL-60. LCLE inhibited cell growth and metabolic activity of the leukemic cells and showed characteristic features of apoptosis. Flow cytometric analysis showed appreciable number of cells in early and late apoptotic stages. While U937 and K562 cell populations...