Extracorporeal shockwave lithotripsy (ESWL) of gall bladder stones leaves residual fragments that need to be dissolved by chemical solvents. In this study we compared the in vitro dissolving capacity of methyl tert-butyl ether (MTBE), mono-octanoin, limonene, and limonene/mono-octanoin (70%/30%). From nine sets of five human gall stones obtained at cholecystectomy, four stones were used for dis...

Plant essential oils (EOs) and a wide range of their individual components are involved in a variety of biological interactions with insect pests including stimulatory, deterrent, toxic and even hormetic effects. Both the beneficial and toxic properties of citrus EOs on the Mediterranean fruit fly (medfly) have been experimentally evidenced over the last years. However, no information is availa...

BACKGROUND The burden of liver disease in the UK has risen dramatically and there is a need for improved diagnostics. AIMS To determine which breath volatiles are associated with the cirrhotic liver and hence diagnostically useful. METHODS A two-stage biomarker discovery procedure was used. Alveolar breath samples of 31 patients with cirrhosis and 30 healthy controls were mass spectrometric...

The methanol extract of Salvia macrochlamys Boiss. and Kotschy was fractionated on a silica gel column to yield a group of terpenic compounds. After separation and cleaning, seven known and three new terpenoid compounds were isolated, and their structures were elucidated by spectroscopic methods, including intensive NMR and MS studies. The crude extract was tested in five different systems for ...

The work presented in this thesis aims to improve the possibilities for diagnosing contact allergy to oxidized limonene and linalool and to, in patients, study contact allergy from structurally closely related hydroperoxides with regard to their specificity, potency and cross-reactivity. The fragrance terpenes limonene (from citrus oil) and linalool (from lavender oil) are widely used in househ...

Th is study was undertaken to investigate the antifungal eff ect of DL-limonene on yeasts using disk diff usion and the minimum inhibitory concentration (MIC). Th e eff ect of DL-limonene on ethyl alcohol fermentation by Saccharomyces cerevisiae was also investigated. According to disk diff usion results, the development of an inhibition zone was observed in all microorganisms tested. Th e inhi...

The plant terpenoids limonene (C10H16) and α-bisabolene (C15H24) are hydrocarbon precursors to a range of industrially relevant chemicals. High-titer microbial synthesis of limonene and α-bisabolene could pave the way for advances in in vivo engineering of tailor-made hydrocarbons, and production at commercial scale. We have engineered the fast-growing unicellular euryhaline cyanobacterium Syne...

The objective of this study was to assess the limonene removal efficiency of three pre-treatment methods when applied to citrus waste and to evaluate their effects on the biochemical methane potential and the methane production rate using batch anaerobic tests. The methods tested were based on removal (biological pretreatment by fungi) or recovery (steam distillation and ethanol extraction) of ...