background: cholera, an infectious disease caused by vibrio cholerae , is primarily transmitted by ingestion of contaminated food or water. in severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death.   methods: in this study v.cholerae non-o1 was cultured in suitable media. lps was extracted from the surface of  bacteria by hot pheno...

conclusions purified lps can be considered as a safe adjuvant and can be used as a component in prophylactic and therapeutic vaccines targeting infectious disease, cancer and allergies. results biochemical analysis data and sds-page profile showed that the chemical nature of s19 lps is different from rb51 lps. both s and r-lps induce an immune response. t-cell immune response induced by both s ...

To study the protective effect of curcumin on lipopolysaccharide-induced rat cardiomyocyte hypertrophy is main objective study. Cardiomyocytes were purified and cultured by differential adherence in vitro then they divided into control group, lipopolysaccharide lipopolysaccharide+curcumin low-dose (12.5 mg/l) medium-dose (25 group high-dose (50 group. The morphological changes observed under a ...

THE EFFECT OF CROCIN ON LIVER DYSFUNCTION INDUCED BY LIPOPOLYSACCHARIDE/D-GALACTOSAMINE IN MALE MOUSE

Alcohol abuse is a common phenomenon among the countries of European continent. One first organs suffering from alcohol-induced damage liver. Activation Kupffer cells, as part mononuclear phagocyte system, plays significant role in development oxidative-nitrosative Systemic inflammatory response affects polarization macrophages throughout body and may affect alcohol hepatocytes. The aim this wo...

Evaluating the Effect of Kaempferol on Sheep Oocyte In Vitro Developmental Competence in Response to Inflammatory Effects Induced by Lipopolysaccharide

The effect of lipopolysaccharide on doxorubicin-induced cell death was studied by using mouse RAW 264.7 macrophage cells. Pretreatment with lipopolysaccharide at 10 ng/mL prevented doxorubicin-induced cell death and the inhibition was roughly dependent on the concentration of lipopolysaccharide. Posttreatment with lipopolysaccharide for 1 hour also prevented doxorubicin-induced cell death. Lipo...

In inflammatory bowel diseases, interleukin-1β production is accelerated. Butyrate, a short chain fatty acid, plays an important role in inflammatory bowel diseases. We investigated the effect of butyrate on interleukin-1β production in macrophage and elucidated its underlying mechanism. We stimulated THP-1 cells, a human premonocytic cell line, by lipopolysaccharide alone and by butyrate with ...

The components of the uridine diphosphate galactose: lipopolysaccharide a-3-galactosyltransferase system from Salmonella iyphimurium were studied. Binary complexes (lipopolysaccharide-phospholipid) and ternary complexes (enzyme-lipopolysaccharide-phospholipid), previously postulated as intermediates in lipopolysaccharide biosynthesis, were reconstituted from the purified components. The complex...