Received October 5, 1970 SUMMARY Lipoxygenase (E.C. 1.99.2.1) from soybean catalyzes its own destruction in the presence of both substrates, oxygen and fatty acid. The apparent first order rate constant for this inactivation was 0.050 min-1 in the presence of 9,12-octadecadienoate and 0.32 min-1 with 5,8,11,14-eicosatetraenoate. Lipoxygenase is a dioxygenase capable of stereospecific attack (1)...

We examined the characteristics of an arachidonate 12-lipoxygenase in bovine tracheal epithelial cells in relation to the enzyme expressed in leukocytes and platelets. Homogenous preparations of intact or disrupted tracheal epithelial cells metabolized arachidonic acid predominantly to (12S)-hydroxyeicosatetraenoic acid, and subcellular fractionation by differential centrifugation demonstrated ...

We examined the characteristics of an arachidonate 12-lipoxygenase in bovine tracheal epithelial cells in relation to the enzyme expressed in leukocytes and platelets. Homogenous preparations of intact or disrupted tracheal epithelial cells metabolized arachidonic acid predominantly to (12S)-hydroxyeicosatetraenoic acid, and subcellular fractionation by differential centrifugation demonstrated ...

The aim of this study was to evaluate the effect of the carotenoidic saponified extract of Physalis alkekengi sepals (PA) towards the lipoxygenase (LOX) oxidation of linoleic acid. Lipoxygenase activity in the presence of carotenoids, standard and from extract, was followed by its kinetic behaviour determining the changes in absorption at 234 nm. The standard carotenoids used were β-carotene (β...

A simple and sensitive kinetic method is described for the determination of ketoprofen in pure form, pharmaceuticals and biological fluids. The method utilizes an oxidative- coupling reaction based upon oxidation of 3-methyl-2-benzo-thiazolinone hydrazone hydrochloride (MBTH) with Ce(IV) in presence of HCl, where an electrophilic intermediate (diazonium salt of the reagent) is produced, then co...

We explored mined block temporarily holding (MBTH) with enforce fork (MBTH-EF) attacks to understand the effect of selfish mining on winning rate and fairness. The temporary time provides a pool additional begin next early. In this situation, may risk losing block. One method maintain probability held is ensure sends out hash values once it receives message from other pools. analyzed MBTH-EF ev...

OBJECTIVE To study a patient with axonal polyneuropathy due to acute dimethylamine borane (DMAB) intoxication. PATIENT Confusion and drowsiness in the acute stage, followed by cognitive impairments and polyneuropathy, are reported in a chemical factory worker after acute exposure to DMAB. RESULTS Nerve conduction studies indicated axonal polyneuropathy, particularly in the motor nerves. Sur...