Conditions are described for the production of large amounts of an extracellular elastolytic protease by Vibrio vulnificus. The yield of enzyme was maximal during the late exponential growth phase and was stable during the stationary growth phase in a medium composed of 2% Proteose Peptone and 1.5% NaCl. The protease has a molecular weight of ca. 50,500 (estimated by sodium dodecyl sulfate-poly...

The equation of state of tantalum (Ta) has been investigated to 100 GPa and 3,000 K using the first-principles molecular dynamics method. A large volume dependence of the thermal pressure of Ta was revealed from the analysis of our data. A significant temperature dependence of the calculated effective Grüneisen parameters was confirmed at high pressures. This indicates that the conventional app...

A soybean cotyledon cDNA library was screened for clones representing RNAs that were more abundant in cotyledons grown on methionine-supplemented medium than in those grown on unsupplemented (basal) medium. Three isolated clones encoded the Bowman-Birk protease inhibitor (BBPI). RNA gel blot hybridization confirmed that the BBPI mRNA level is increased by methionine (Met). Met and/or sulfate su...