By using immunohistochemistry and in situ hybridization, we have demonstrated that the nitric oxide (NO)-synthesizing enzyme NO synthase is present in gonadotrophs and in folliculo-stellate cells of the anterior pituitary gland of male and female rats. A marked increase in levels of NO synthase protein and mRNA was observed after gonadectomy. In vitro studies on dispersed anterior pituitary cel...

Indeed, optio is Weaver who is credited with the origin of the term molecular biology in 1938. Development of luteinizing hormone releasing hormone neurones.

The glycoprotein hormones of the anterior pituitary and of the placenta are made up of a and p subunits, one of which, the a, is common to each hormone. Thcy therefore share some similar physicochemical and immunological properties, and the complete purification of follicle-stimulating hormone, for instance, has proved difficult. Many preparations which have appeared to be pure by one criterion...

The gonadotropin-ovary axis of patients with anorexia nervosa was assessed with the aim of distinguishing between an intrinsically hypophysial deficiency and pituitary malfunction secondary to hypothalamic impairment. The most consistent endocrine abnormality was low serum luteinizing and follicle stimulating hormone (LH and FSH) levels associated with depressed serum estradiol levels. Gonadotr...

The location of renin (EC 3.4.99.19) in rat pituitary was determined by the peroxidase-antiperoxidase immunohistochemical technique. By using antisera prepared with purified rat renal renin, an immunoreactive substance was localized within ovoid cells scattered throughout the anterior pituitary. These cells were shown to be luteinizing hormone-producing cells by staining with anti-luteinizing h...