isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. among the non-tuberculous mycobacteria, these tests are unable to differentiate m. scrofulaceum from m. avium-intracellulare (mai) because of their identical biochemical characteristics. for this reason, one hundred mycobacterial strains suspected to be m. sc...

Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. ...

Mycobacterium scrofulaceum is an environmental and slow-growing atypical mycobacterium. Emerging evidence suggests that M. scrofulaceum infection is associated with cervical lymphadenitis in children and pulmonary or systemic infections in immunocompromised adults. However, the nature of host innate immune responses to M. scrofulaceum remains unclear. In this study, we examined the innate immun...

Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. sc...

Fluorescent acid-fast microscopy (FAM) was used to enumerate intracellular Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum in the ciliated phagocytic protozoan Tetrahymena pyriformis. There was a linear relationship between FAM and colony counts of M. avium cells both from cultures and within protozoa. The Ziehl-Neelsen acid-fast stain could not be used to enum...

To obtain information on the epidemiology of mycobacteriosis, water and air samples collected along the East Coast of the United States were examined for mycobacteria. Mycobacterium avium-intracellulare-scrofulaceum (MAIS) were isolated from 25% of the water, samples, mostly those from South Carolina, Georgia, and the Gulf states. Mycobacterium kansasii and Mycobacterium marinum were not found,...

A cooperative study was conducted by the International Working Group on Mycobacterial Taxonomy to correlate the agglutination serovar designations of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum strains with the species ascriptions of these organisms according to molecular criteria and cultural properties and to assess the reproducibility of serovar determin...

Modifications to the tube-agglutination procedure which Schaefer developed for serotyping of organisms of the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex are proposed.