To develop a more sensitive immunoassay for malachite green (MG) and leucomalachite green (LMG), we identified the immunocomplex binding phage-borne peptides for use in the noncompetitive phage anti-immunocomplex assay (PHAIA). An anti-LMG monoclonal antibody (mAb) was used to select immunocomplex binding peptides from a circular random eight-amino-acid phage-displayed library. After three roun...

Fluorescein as an Indicator of Antihistaminic Activity: Inhibition of Histamine-induced Fluorescence in the Skin of Human Subjects: Samuel C. Bukantz and Gustave J. Dammin 224 Effect of Hormone on Root Formation in Artocarpus communist: Thomas J. Muzik ...... 225 The Inhibition of Virus Increase by Malachite Green: William N. T kahashi .................................... 226 Stability of Solut...

Titanium dioxide (TiO2) and Fe3O4 magnetite particles were coated on spherical Kissirises; glucose oxidase (GOx) enzyme was immobilized on Kissiris/Fe3O4/TiO2 by physical adsorption. This catalyst was analyzed by a scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray (EDX) measurements. The performance of the prepared biocatalyst in the...

A photometric method for the determination of the acid nucleoside triphosphatase (EC 3.6.1.—) is described, in which inorganic phosphate is liberated from ATP or other nucleoside triphosphates. Colorimetric determination of liberated phosphate is based on the formation of a green complex of phosphomolybdate and malachite green hydrochloride. Optimal test conditions were evaluated as well as the...

Resistant strains of Aspergillus nidulans were obtained by one-step selection on high concentrations of various inhibitors; each strain was mutant in a different gene conferring resistance to actidione (Act), p-fluorophenylalanine (p f ) , teoquil ( te) , iodoacetate (Iod) or malachite green (mg). Some mutant alleles have been firmly, others tentatively, located. For comparative purposes attemp...

Malachite green (MG), N-methylated diaminotriphenylmethane, is one of the most common dyes in textile industry and has also been used as an effective antifungal agent. However, due to its negative impact on the environment and carcinogenic effects to mammalian cells, there is a significant interest in developing microbial agents to degrade this type of recalcitrant molecules. Here, an Exiguobac...

The objective of this study was to investigate the response of Na(+)/K(+)-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm(-2) and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medi...

The gene (CcLcc2) encoding laccase from the basidiomycete Coprinopsis cinerea Okayama-7 #130 was synthesized by polymerase chain reaction-based two-step DNA synthesis, and heterologously expressed in Pichia pastoris. The recombinant protein was purified by ammonium sulphate precipitation and nickel nitrilotriacetic acid chromatography. The molecular mass of CcLcc2 was estimated to be 54 kDa by ...

A simple, environment friendly and efficient technique, ionic liquid-based microwave-assisted extraction was first used to determine malachite green and crystal violet (CV) from water samples coupled to HPLC. The key parameters influencing extraction efficiency were investigated, such as the type of ionic liquids, the volume of ionic liquid, extraction time, and so on. Under the optimum conditi...

The survival of microorganisms of the Mycobacterium avium, M. intracellulare, and M. scrofulaceum (MAIS) complex was evaluated after various soil and water decontamination regimens. Survival was reduced by growing cells in natural waters compared with laboratory media and by inclusion of malachite green in media as an antifungal agent. Decontamination with benzalkonium chloride, while reducing ...