A facile method for accurate detection of type 1 dengue virus (DV1) infection from complex biological mixtures, using type specific immunocapture coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed. The biological mixtures were treated with magnetic particles coated with a monoclonal antibody directly against type 1 dengue viru...

Phage amplification detected by MALDI-TOF MS was investigated for rapid and simultaneous Burkholderia pseudomallei identification and ceftazidime resistance determination. B. pseudomallei ceftazidime susceptible and resistant ΔpurM mutant strains Bp82 and Bp82.3 were infected with broadly targeting B. pseudomallei phage ϕX216 and production of the m/z 37.6 kDa phage capsid protein observed by M...

BACKGROUND Protein profiling with high-throughput sample preparation and MALDI-TOF MS analysis is a new potential tool for diagnosis of human diseases. However, analytical reproducibility is a significant challenge in MALDI protein profiling. This minireview summarizes studies of reproducibility of MALDI protein profiling and current approaches to improve its analytical performance. METHODS T...

Candidemia is a health problem with high mortality rate. The aims of this study were to compare the identification Candida spp. via traditional methodologies and mass spectrometry (MALDI-TOF/MS) in order verify antifungal susceptibility profile these isolates analyze clinical evolution patients. This was two-year prospective cohort conducted at Hospital de Clínicas Uberlândia (HCU-UFU). identif...

Acinetobacter species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid reliable identification, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was applied from The aims the study were, thus, identify profile tissues fish living vulnerable environment impacted by ...

background: culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. methods: in this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. protein profile of recombinant cho (rcho) ce...