To establish a proteomic reference map for soybean leaves, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 260 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS. Fifty-three of these protein spots were id...

in this study, 120 accessions of aegilops crassa collected from various geographical areas of iran were analyzed with respect to genome size and protein markers. a flow cytometry survey of these accessions revealed that one hundred and thirteen of the accessions were tetraploid and seven were hexaploid. moreover, these accessions revealed variations in high molecular weight glutenin subunit com...

A brief introduction is presented to the basic principles and application of a quadrupole-time-of-flight (TOF) tandem mass spectrometer. The main features of reflecting TOF instruments with orthogonal injection of ions are discussed. Their operation and performance are compared with those of triple quadrupoles with electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) ...

A method of direct lipid analysis by MALDI mass spectrometry in intact membranes, without prior extraction/separation steps, is described. The purple membrane isolated from the extremely halophilic archaeon Halobacterium salinarum was selected as model membrane. Lyophilized purple membranes were grinded with 9-aminoacridine as dry matrix and the powder mixture crushed in a mechanical die press ...

Glycomics is a developing field that provides structural information on complex populations of glycans isolated from tissues, cells and organs. Strategies employing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) are central to glycomic analysis. Current MALDI-based glycomic strategies are capable of efficiently analyzing glycoprotein and glycosphingo...

Circulating atypical cells (CAC) are released from a primary tumour site into peripheral blood and indicators of cancer metastasis. CAC occur at very low frequency in circulating blood, their detection remains challenging. Moreover, white (WBC) the major contaminant enriched samples. Here, we developed matrix-assisted laser desorption ionization–time flight mass spectrometry (MALDI-TOF MS) as n...

Multilocus sequence typing (MLST) is an important tool for the global surveillance of bacterial pathogens that is performed by comparing the sequences of designated housekeeping genes. We developed and tested a novel mass spectrometry-based method for MLST of Streptococcus pneumoniae. PCR amplicons were subjected to in vitro transcription and base-specific cleavage, followed by analysis of the ...