The goal of this study was to test the relationship between malonyl-CoA concentration and its turnover measured in isolated rat hearts perfused with NaH(13)CO(3). This turnover is a direct measurement of the flux of acetyl-CoA carboxylation in the intact heart. It also reflects the rate of malonyl-CoA decarboxylation, i.e. the only known fate of malonyl-CoA in the heart. Conditions were selecte...

malonyl-coa decarboxylase (mcd) is an enzyme involved in the decarboxylation of malonyl-coa to acetyl-coa. in order to explore the hypothesis that the changing plant materials’ mcd activity level can serve as therapy to diabetics, the effect of teucrium polium compounds was studied in a diabetic rat model. in this experimental study, two groups of rats, a control and a diabetic group, each incl...

Malonyl-CoA decarboxylase was purified from goose uropygial gland, reduced, carboxymethylated, and digested with trypsin. Several peptides were purified by high performance liquid chromatography and their amino acid sequences determined. Oligonucleotide probes were prepared based on their amino acid sequences. Size-selected RNA from the goose uropygial gland was used to construct cDNA libraries...

background the malonyl-coa decarboxylase (mcd, ec.4.1.1.9) enzyme regulates malonyl-coa levels. the effect of aerial parts extracts of urtica dioica (ud) on mcd is poorly understood. objectives the present experiment was undertaken to evaluate the effect of ud aerial parts extracts on mcd level. materials and methods in this experimental study, two groups of rats were used: normal and hyperglyc...

F atty a cid s ynthase ( FAS) inhibition initiates selective ap optosis o f can cer cel ls b oth in vivo and in vitro, which m ay i nvolve malonyl-CoA metabolism. T hese findings l ed to exploration of malonyl-CoA decarboxylase ( MCD) as a p otential novel target for cancer treatment. M CD regulates the levels of cellular malonyl-CoA through the decarboxylation of malonyl-CoA to acetyl-CoA. Mal...

Anaerobic decarboxylation of malonate to acetate was studied with Sporomusa malonica, Klebsiella oxytoca, and Rhodobacter capsulatus. Whereas S. malonica could grow with malonate as sole substrate (Y = 2.0 g.mol-l) , malonate decarboxylation by K. oxytoca was coupled with anaerobic growth only in the presence of a cosubstrate, e.g. sucrose or yeast extract (Y, = 1.1-1.8 g.mol malonate-1). R. ca...

Malonyl-CoA decarboxylase (E.C.4.1.1.9) catalyzes the conversion of malonyl-CoA to acetyl-CoA. Although the metabolic role of this enzyme has not been fully defined, it has been reported that its deficiency is associated with mild mental retardation, seizures, hypotonia, cadiomyopathy, developmental delay, vomiting, hypoglycemia, metabolic acidosis, and malonic aciduria. Here, we isolated a cDN...

Lipid metabolism is highly controlled by the nutritional state of the organism. In this study, we identify the mitochondrial sirtuin, SIRT4, as a critical regulator of lipid homeostasis. We find that SIRT4 represses fatty acid oxidation while promoting lipid anabolism. Mechanistically, SIRT4 regulates this balance by inhibiting malonyl-CoA decarboxylase (MCD), an enzyme that produces acetylCoA ...

1. Rat soleus strips were incubated with 5 mM glucose, after which tissue metabolites were measured. Alternatively, muscle strips were incubated with 5 mM glucose and 0.2 mM palmitate, and the formation of 14CO2 from exogenous palmitate or from fatty acids released from prelabelled glycerolipids was measured. 2. Etomoxir, which inhibits the mitochondrial overt form of carnitine palmitoyltransfe...

Obesity is an important contributor to the risk of developing insulin resistance, diabetes, and heart disease. Alterations in tissue levels of malonyl-CoA have the potential to impact on the severity of a number of these disorders. This review will focus on the emerging role of malonyl-CoA as a key "metabolic effector" of both obesity and cardiac fatty acid oxidation. In addition to being a sub...