نتایج جستجو برای: mashhadpcrseroindeterminatewestern blot
تعداد نتایج: 45661 فیلتر نتایج به سال:
Chemiluminescent western blotting has been in common practice for over three decades, but its use as a quantitative method for measuring the relative expression of the target proteins is still debatable. This is mainly due to the various steps, techniques, reagents, and detection methods that are used to obtain the associated data. In order to have confidence in densitometric data from western ...
گزنه دو پایه (urtica dioica l.)، دارای خاصیت درمانی در معالجه بیماریهای مزمن انسان می باشد. در این پژوهش اثر ضد ویروسی عصاره های آبی و الکلی گزنه در مهار ویروسهای بیماری زای ایجاد کننده موزائیک گل رُز در محیط کشت مورد بررسی قرار گرفت. تعداد 530 نمونه از گونه های مختلف رُز دارای علائم موزائیک و یا بدون علائم از مناطق محتلف تهران و حومه جمع آوری شد. نمونه ها توسط آزمونهای سرولوژیک الیزای مستقیم و غ...
streptococcus iniae is a major cause of serious bacterial infections in both fish and human beings. capsular polysaccharide (cps) of s. iniae is vital to evade phagocytic clearance of the host and serves as an important protective antigen of s. iniae infection in aquatic animals. the cpsdgene was determined to be highly conservative in capsule polysaccharide operon. prokaryotic expression of th...
nucleocapsid (n) protein of infectious bronchitis virus (ibv), one of the viral structural proteins, inducesstrong antibody response in natural infection. in this study, a simple, recombinant n protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of ibv antibody.initially, 72 serum samples were tested for the presence of ibv antibody using a c...
مقدمه: cryptosporidiosis یکی از علل مهم اسهال در انسان و حیوانات می باشد. تشخیص این بیماری، بر پایه ی استفاده از روش های میکروسکوپی با حساسیت پایین است. هدف از انجام این مطالعه، استفاده از روش dot blot جهت شناسایی انگل بود. روش ها: 131 نمونه ی مدفوع از گوساله های شیرخوار جمع آوری شد و با استفاده از روش فرمالین اتر، روی آن ها تغلیظ انجام گرفت. سپس، نمونه ها به روش ziehl-neelsen اصلاح شده، رنگ آم...
abstract: potato virus y a type species of the genus potyvirus infects several crops in the family solanaceae. pvy isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. the result of elisa, immunoblot electrophoresis and immuno capture reverse transcription polymerase chain reaction (ic-rt-pcr) indicated that pepper isol...
objective dot blot (db) assay provides highly specific results, but usually not reliable for quantification of antibody production. the need for a more objective db assay to provide a better definition of the immune status, against hiv antigens, promoted this study to be done to develop a quantitative db assay. material and methods dot blot (db) strips for antibodies directed to human immunodef...
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Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...
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