Orbitrap is the newest addition to the family of high-resolution mass spectrometry analyzers. With its revolutionarily new, miniature design, Orbitrap combines high speed with excellent quantification properties, ranking favorably in many analytical applications.

We have developed a technique to simultaneously compare the cyclotron frequencies of two single ions to obtain atomic mass ratios with fractional accuracies at or below 10−11. Much like a balance scale, this two-ion technique cancels many sources of noise and error – chief among them magnetic field noise. The new mass comparisons are used in combination with γ-ray wavelength measurements, perfo...

The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies. Unlike the HPLC method, a substantially larger amount (~10‐20 times) of glycan is normally required for a single MS spectrum. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry i...

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In single-molecule mass spectrometry, the mass of each ion is measured individually; making it suitable for the analysis of very large, heterogeneous objects that cannot be analyzed by conventional means. A range of single-molecule mass spectrometry techniques has been developed, including time-of-flight with cryogenic detectors, a quadrupole ion trap with optical detection, single-molecule Fou...

Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS) enables the visualization of the distribution of a range of biomolecules that have varied structures in the cells and tissue sections. This emerging imaging technique was initially developed as a tool for protein imaging, but recently it is increasingly being used for the imaging of small organic molecules. IMS ...

Common to all experiments in mass spectrometry is the creation of gas-phase ions. Electron impact (EI) ionization is a familiar method for creating ions from volatile gas-phase molecules (M). In EI ionization, fast-moving electrons remove an electron from the neutral molecule to create the odd-electron molecular ion M`Õ. Another common ionization method is chemical ionization (CI). Here, an ion...