Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...

Apple is one of the most important temperate-zone fruit trees in the world. Micropropagation of apple scion and rootstocks is an important method of rapid production of healthy and disease-free plants. In this research, in vitro propagation of three apple roottocks including Azayesh-Esfahan, Morabbaee-Mashhad and M9 was investigated. Shoot proliferation carried out in two basal media (MS and WP...

a methodology to regenerate whole plants of fig tree (ficus carica cv. jaami-e-kan) from apical meristem of greenhouse plantlets was developed. two meristematic explants size (0.2-0.4 and 0.5-0.7 mm), two kinds of culture media and three concentrations of 6-benzyladenine (0.5, 1 and 1.5 mgrl-1) were tested as a completely randomize design (crd) with a simple factorial arrangement for establishm...

introduction: potential almond rootstock and thus main genetic sources for the breeding of its new rootstocks are almond itself, peach and plum, and these species cross-breeds almond is a source of resistance to limestone and to drought because of its roots, which reach deep layers of soil; it is also more resistant than peach to na and bo. asexual or vegetative propagation is a hereditable cha...

An efficient in vitro protocol was standardized for Almond (Prunus dulcis) propagation using dormant axillary buds as explants. Explants were cultured on Murashige and Skoog (MS) and woody plant medium (WPM) supplemented with different concentration/combination(s) of phytohormones. MS basal medium showed lowest shoot induction and took longest duration for shoot initiation. Multiple shoots were...

Seeds of Bauhinia acuminata L. were used as an experimental material for micropropagation. The effect of growth regulators concentrations on morphogenetic development was studied. Seeds were germinated on MS medium supplemented with GA3 (0.10, 1.0 and 2.0 mgL -1 ) and without GA3. Satisfactory germination was observed at media with 1.0 mgL -l GA3. Subsequent propagation from plantlet was perfor...

in order to micropropagate of four vigorous rootstocks of pear, including pyrus betulifolia, konjuni, dargazi and gh1, effects of basic plant culture media (ms, ql and modified ql), cytokinins and auxins on productivity, proliferation and rooting were studied in seed and plant improvement institute, karaj. the highest amount of productivity for all rootstocks was observed in ql medium. among pe...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL(-1)) solely or in combination with cytokinins BAP and K...