The emergence and spread of the mobile colistin resistance gene (mcr-1) has become a major global public health concern. So far, this gene has been widely detected in food animals, pets, food, and humans. However, there is little information on the contamination of mcr-1-containing bacteria in farming soils. In August 2016, a survey of ESBL-producing Escherichia coli isolated from farming soils...

Since the initial discovery of mcr-1 in an Escherichia coli isolate from China, the gene has also been detected in Klebsiella pneumoniae and Salmonella enterica but is rarely reported in other Enterobacteriaceae Here, we report the isolation and identification of a Shigella flexneri strain harboring mcr-1 from stool samples in a pig farm in China from 2009. The MIC of colistin for the isolate i...

Methyl-coenzyme M reductase (MCR) is a nickel tetrahydrocorphinoid (coenzyme F430) containing enzyme involved in the biological synthesis and anaerobic oxidation of methane. MCR catalyzes the conversion of methyl-2-mercaptoethanesulfonate (methyl-SCoM) and N-7-mercaptoheptanoylthreonine phosphate (CoB7SH) to CH4 and the mixed disulfide CoBS-SCoM. In this study, the reaction of MCR from Methanot...

background: amyotrophic lateral sclerosis (als) is the most common motor neuron disorder in european populations. als can be sporadic als (sals) or familial als (fals). among 20 known als genes, mutations in c9orf72 and superoxide dismutase 1 (sod1) are the most common genetic causes of the disease. whereas c9orf72 mutations are more common in western populations, the contribution of sod1 to al...

The origin and mobilization of the ~2,609-bp DNA segment containing the mobile colistin resistance gene mcr-1 continue to be sources of uncertainty, but recent evidence suggests that the gene originated in Moraxella species. Moreover mcr-1 can be mobilized as an ISApl1-flanked composite transposon (Tn6330), but many sequences have been identified without ISApl1 or with just a single copy (singl...

Mucormycosis (MCR) involving paranasal sinuses is a rare life-threatening opportunistic infection in immunocompromised individuals. In humans, MCR considered as one of the most rapidly progressive lethal forms fungal with high mortality rate 70–100%. During second wave COVID-19 India, cases have increased rapidly. We herein report case 70-year-old Indian male an uncontrolled diabetes diagnosed ...

Plasmid-mediated colistin-resistance genes have been reported worldwide in recent years. A multiplex polymerase chain reaction (Multi-PCR) protocol was developed to detect transferable colistinresistance ( mcr-1 mcr-6 ) Enterobacteria for clinical laboratory purposes.The authors first designed six new primer pairs amplify gene products achieve stepwise separation of amplicons between 87 216 bp,...

A temperature insensitive three-turn microfiber coil resonator (MCR) is demonstrated by embedding it in Teflon with opposite thermo-optic coefficient. The temperature dependence of a MCR strongly depends on the microfiber size which controls the ratio of thermal effect contributions from silica and polymer. Fabricated from a ~3μm-diameter microfiber, the temperature dependence of our MCR is com...