Recent advances in fluorescence spectrophotometry enable the analysis of river dissolved organic matter. We investigate the potential of detecting sewage pollution in a small, urbanised catchment. Downstream sampling highlighted a summer maximum in tryptophan fluorescence intensity during low flow. No correlation is observed between ammonia and tryptophan fluorescence intensity. In contrast, tw...

Blood contamination, such as bloody ascites or hemorrhages during surgery, is a potential hazard for clinical application of fluorescence imaging. In order to overcome this problem, we investigate if fluorescence-lifetime imaging helps to overcome this problem. Samples were prepared at concentrations ranging 0.3-2.4 μm and mixed with 0-10% of blood. Fluorescence intensities and lifetimes of sam...

In fluorescence fluctuation polarization sensitive experiments, the limitations associated with detecting the rotational timescale are usually eliminated by applying fluorescence correlation spectroscopy analysis. In this paper, the variance of the time-averaged fluorescence intensity extracted from the second moment of the measured fluorescence intensity is analyzed in the short time limit, be...

Present: Due to an error in the production process, figures 5 and 6 were switched. Each figure legend is correct, but is associated with the wrong figure. Corrected: The proper figure legends are provided below. The publisher sincerely apologizes for this oversight.alpha [500 U/ml] stimulation. MFI = mean relative fluorescence intensity. *indicates significant difference to untreated controls. ...

Fluorescence lifetime-resolved images of chlorophyll fluorescence were acquired at the maximum P-level and during the slower transient (up to 250 s, including P-S-M-T) in the green photosynthetic alga Chlamydomonas reinhardtii. At the P-level, wild type and the violaxanthin-accumulating mutant npq1 show similar fluorescence intensity and fluorescence lifetime-resolved images. The zeaxanthin-acc...

Fluorescence imaging is a widely adopted technique in various scientific disciplines for mapping chemical and biological characteristics or molecular interactions in cells by generating high contrast images in a non-invasive way. Recently, fluorescence lifetime imaging microscopy (FLIM) has become a practical alternative to fluorescence intensity thanks to affordable pulsed laser sources and co...

UNLABELLED The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well Nd...

BACKGROUND Lens autofluorescence increases with the age of the subject, and the fluorophores responsible are associated with cataract, retinopathy, and other complications of diabetes. We built a scanning confocal lens fluorescence biomicroscope suitable for routine clinical measurement of lens autofluorescence and light scattering and report data from 127 healthy subjects. METHOD The fluores...

BACKGROUND Human leukocyte antigen (HLA)-DQ has emerged as the alloantibody most frequently associated with the generation of de novo donor-specific antibody (DSA), antibody-mediated-rejection, and unfavorable transplantation outcome. METHODS The generation of HLA-DQ de novo DSA was interrogated in 40 transplant recipients who were immunologically naive before their failed transplantation. Ep...

Flow cytometry has been used to detect estrogen receptor (ER) in intact cells, using 1-(N)- fluoresceinyl estrone thiosemicarbazone ( 17FE ), a ligand shown to have sufficient affinity and specificity to identify high-affinity receptors. For each concentration of 17FE , two fluorescent distributions were obtained: (a) "total" fluorescence due to 17FE alone; and (b) diethylstilbestrol-inhibited ...