(S3) Measurement of two-photon cross section. (S3) Cytotoxicity assay. (S3) Cell culture. (S4) Two-photon and one-photon fluorescence bioimaging. (S4) Calculation of mean fluorescence intensity (S4) Table S1. Absorption and emission maxima of 1a~1c in DMSO. (S4) Table S2. Absorption and emission maxima of 1c in various solvents. (S10) Fig. S10. 2P microscopy of 2a-labeled HeLa cells for biothio...

A new sensitive, simple and rapid method for determination of methotrexate (MTX) was developed based on quenching effects of MTX on the fluorescence intensity of Tb3+-1,10-phenanthroline complex. The fluorescence intensity was measured with excitation wavelength of 300 nm and emission wavelength of 545 nm and the quenched fluorescence intensity is proportional to the concentration of MTX in Tris...

A new sensitive, simple and rapid method for determination of methotrexate (MTX) was developed based on quenching effects of MTX on the fluorescence intensity of Tb3+-1,10-phenanthroline complex. The fluorescence intensity was measured with excitation wavelength of 300 nm and emission wavelength of 545 nm and the quenched fluorescence intensity is proportional to the concentration of MTX in Tris...

The long-term preservation of fluorescence qualities resin-based composite (RBC) restorations is an absolute condition for the implementation fluorescence-aided identification technique (FIT) in dentistry and forensic medicine. Therefore, this study assessed 244 color shades 16 commercially available RBC brands with a monochromator-based multimode microplate reader. specimens were stored dark a...

Vol. 11 | No. 03 | May June 2014 | UROLOGY JOURNAL Donor Specific Antibodies Median Fluorescence Intensity Levels Are the Best Indicator for Monitoring Desensitization Treatment in Kidney Transplant Francisco Boix,1 Santiago Llorente,2 Anna Mrowiec,1 Jorge Eguia,1 Ruth López-Hernández,1 Maria Victoria Bernardo,1 María Rosa Moya-Quiles,1,3 José A. Campillo,1,3 Alfredo Minguela,1,3 Luisa Jimeno,2...

Several methods have been proposed for comparing fluorescence intensity curves obtained from flow cytometry analysis. The one comparison method that is most often used is the Kolmogorov-Smirnov (K-S) test, which is known to find statistically significant differences between curves even when clinical or scientific differences are doubtful. In the field of flow cytometry, the combination of the g...

Three probes were evaluated for their effectiveness in measuring connective tissue fluorescence within carcasses or primal cuts: 1) a quartz-glass rod, 2) a light guide formed from bundles of optical fibers, and 3) a single optical fiber. The shape of the fluorescence emission spectrum of tendon was altered by the method of measurement, probably because of differences in the intensity of excita...

Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure fluorescence spectra at different calibrated levels of pressure was designed a...