a new sensitive, simple and rapid method for determination of methotrexate (mtx) was developed based on quenching effects of mtx on the fluorescence intensity of tb3+-1,10-phenanthroline complex. the fluorescence intensity was measured with excitation wavelength of 300 nm and emission wavelength of 545 nm and the quenched fluorescence intensity is proportional to the concentration of mtx in tris...

The genetic and phenotypic heterogeneity of cancers can contribute to tumor aggressiveness, invasion, and resistance to therapy. Fluorescence imaging occupies a unique niche to investigate tumor heterogeneity due to its high resolution and molecular specificity. Here, heterogeneous populations are identified and quantified by combined optical metabolic imaging and subpopulation analysis (OMI-SP...

in this study, the fluorogenic efficiency of fluorescamine for spectrofluorimetric analysis of primary amines was investigated. different mixtures of dichloromethane and other organic solvents were used for evaluation of fluorescence stability and intensity of fluorescamine derivative (pyrrolinone). solvents such as acetonitrile (acn), tetrahydrofuran (thf) and dioxane increase stability and in...

PURPOSE It has been reported that a dysfunction of T lymphocytes can be responsible for alteration in immune system in patients with systemic lupus erythematosus (SLE). MATERIAL AND METHODS Using flow cytometric analysis, we determined the abnormalities of T cell receptor zeta (TCR zeta) chain contents in CD4+ T cells of SLE patients. RESULTS We observed a decrease in mean fluorescence inte...

OBJECTIVE We aimed to clarify the optimal timing for the fluorescence imaging of brain tumor tissue differentiated from brain edema after the administration of photosensitizers. METHODS We have performed an in vivo study of the kinetics of 5-aminolevulinic acid (5-ALA) in comparison with talaporfin sodium using the rat brain tumor model and rat vasogenic edema model produced by cold injury. T...

background: inappropriate activation or blockage of the inhibition of complement system could cause tissue damages in autoimmune diseases particularly rheumatoid arthritis (ra). defect in complement component regulation may cause damages to tissues, on the other hand, or the damaged tissue might affect the unnecessary activation of complement components.   objective: to investigate the expressi...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 206 No. 3 395–413 www.jcb.org/cgi/doi/10.1083/jcb.201403039 JCB 395 Correspondence to Christopher E. Turner: [email protected] Abbreviations used in this paper: apFRET, acceptor photobleaching FRET; CDM, cell-derived matrix; co-IP, coimmunoprecipitation; FA, focal adhesion; FRET, fluorescence resonance energy transfer; HDAC, histone ...

Cationic dye Acridine orange (AO) has wide applications especially in biologicalfields such as analysis of lysosomal and mitochondria content by flow cytometryand so on. In the current work, spectroscopy of acridine orange (AO) dye at bothlow concentrations (mdye/mwater=6.25×10-5, 3.12×10-5) and high concentrations(mdye/mwater=0.002, 0.001) was studied in confined water ...

OBJECTIVE To explore the fluorescence characteristics of common cariogenic bacteria: Streptococcus mutans, S. sanguis, Actinomyces viscosus, Prevotella intermedia, Lactobacillus acidophilus, and Candida albicans. METHODS The bacteria were cultured on brain heart infusion (BHI) agar and BHI blood agar, and bacterial colonies were collected for further amplification in liquid medium. Bacterial ...

A quantitative empirical model is presented, which relates the total surfactant concentration with the fluorescence intensity and the mean translational diffusion coefficient of dyes in micellar solutions, as determined by Fluorescence Correlation Spectroscopy (FCS). Based on this model a systematic data analysis method is defined for the precise determination of the dye binding equilibrium con...