IT has been lOyr since the initial randomized placebo-controlled trials were published which established the short-term efficacy of low-dose weekly methotrexate in rheumatoid arthritis [1,2]. For the past 10 yr there has been an extensive clinical research programme including comparative studies of methotrexate versus auranofin [3], azathioprine [4], i.m. gold [5] and cyclosporin A [6], and lon...

background: induced abortion is the medical or surgical termination of pregnancy before fetal viability. it has maternal or fetal indications. the aim of the present study was to evaluate the efficacy of the combination of methotrexate and misoprostol for termination of the pregnancy in the first trimester.   methods: this analytic study was performed on 100 women at the first trimester of preg...

We examined the pharmacological role of the renal organic anion transporter OAT-K1, which localizes predominantly in the brush-border membranes of proximal straight tubules, in the urinary excretion of methotrexate and the possibility of its contribution to "folinic acid rescue." With Madin-Darby canine kidney (MDCK) cells stably transfected with OAT-K1 cDNA, OAT-K1-mediated methotrexate accumu...

Previous investigations have suggested that high-dose methotrexate with leucovorin rescue is a potentially useful strategy for overcoming antifolate resistance. Interactions between methotrexate (MTX) and leu covorin and their respective metabolites appear to occur at multiple intracellular sites, including dihydrofolate reducÃ-ase(MTX/MTX polyglutamates versus dihydrofolate) and other folate-d...

OBJECTIVE In a real-life setting, to analyse retrospectively the effects of different methotrexate regimens on etanercept efficacy during the first year of treatment for rheumatoid arthritis (RA). METHODS Demographic characteristics, clinical parameters and treatment data from patients with RA receiving the first-line biological disease-modifying antirheumatic drug, etanercept, as monotherapy...

Life-threatening drug interactions are known to occur between methotrexate and nonsteroidal anti-inflammatory drugs (NSAIDs), probenecid, and penicillin G. The purpose of this study was to characterize methotrexate transport, as well as to determine the site and the mechanism of drug interactions in the proximal tubule. Mouse proximal tubule cells stably expressing basolateral human organic ani...

Methotrexate, a structural analogue of folic acid, is one of the most frequently used chemotherapeutics, especially in haematological malignancies, various solid tumours and also inflammatory disorders. Methotrexate interferes with folate metabolism, mainly by inhibition of dihydrofolate reductase, resulting in the suppression of purine and pyrimidine precursor synthesis. The depletion of nucle...

OBJECTIVE The relative high cost and potential side effects mandate careful scrutiny as to when tumor necrosis factor alpha (TNF) inhibitors should be used in everyday practice. We surveyed how TNF inhibitors performed in randomized controlled trials when compared to methotrexate in methotrexate naive rheumatoid arthritis patients. METHODS We identified all randomized controlled trials with T...

Methotrexate is a cytostatic drug applied in adjuvant chemotherapy and associated with cognitive impairment in part of the cancer patients. In this paper we studied in rats whether a reduction in blood supply to the brain or neuroinflammation are possible mediators of this cognitive dysfunctionality. Methotrexate reduced hippocampal blood vessel density 1 week and 3 weeks after treatment as mea...

[8H]Methotrexate was metabolized mainly to 4-amino-4deoxy-lo-methylpteroic acid in resting cells of ZactobaciZZus casei and a small amount of methotrexate remained inside the cells after 24 hours of incubation. Preincubation of L. casei with methotrexate inhibited the incorporation of [3H]folic acid completely and I-5-formyl-[3H]tetrahydrofolic acid partially into pteroyloctaglutamate and incre...