Dermal microdialysis was used to assess the bioavailability of a topical corticosteroid, clobetasol propionate, following application onto the skin of human subjects. The penetration of clobetasol propionate from a 4% m/v ethanolic solution applied onto 4 sites on one forearm of healthy human volunteers was studied. A lipid emulsion, Intralipid®, was used as the perfusate and linear microdialys...

Electrochemistry Discover the difference Summary The ALEXYS Neurotransmitter Analyzer is a modular system for UHPLC with electrochemical detection of neurotransmitters. This application note shows the applicability of the system to measure various biogenic amines in on-line microdialysis (OMD) which is a continuously flowing microdialysis sampling setup. Instead of collecting samples and puttin...

Pharmacokinetic investigations of antibiotics in peripheral tissue are important for appropriate and effective treatment of infections and for prophylactic use. Bone tissue is difficult to harvest, handle and analyse. The aim of this PhD dissertation was to apply microdialysis to bone tissue for the investigation of gentamicin in bone tissue. Microdialysis probes consist of thin semi-permeable ...

Drug addiction has developed to a social illness. Changes in glutamate transmission have been recorded by the repeated administration of addictive drugs into VTA. In this investigation, In vivo microdialysis was used to study the effects of morphine on glutamate release from the ventral tegmentum area (VTA) in freely moving rats. Rats were anesthetized with chloral hydrate (350 mg/kg, i.p.) and...

Cerebral microdialysis is a sampling technique which offers much potential for understanding inflammatory pathophysiology following traumatic brain injury (TBI). At present, the recovery of cytokines via microdialysis in clinical studies is not straightforward primarily due to their size, steric properties and low concentrations. Heparin and heparin-coated microspheres have previously shown pro...

INTRODUCTION The assessment of adrenal function in critically ill patients is problematic, and there is evidence to suggest that measurement of tissue glucocorticoid activity may be more useful than estimation of plasma cortisol concentrations. Interstitial cortisol concentrations of cortisol represent the available pool of glucocorticoids able to enter the cell and bind to the glucocorticoid r...

Clinical microdialysis allows a discrete volume of the brain to be sampled for neurochemical analysis of neurotransmitters, metabolites, biomarkers, and drugs. The technique can be safely used in humans intraoperatively, in the intensive care unit, and in ambulatory settings. Microdialysis probes, micropumps, and analytical equipment are commercially available and have been used extensively for...

In vivo intracerebral microdialysis is an important neurochemical technique that has been applied extensively in genetic and pharmacological studies aimed at investigating the relationship between neurotransmitters. Among the main interests of microdialysis application is the infusion of drugs through the microdialysis probe (reverse dialysis) in awake, freely moving animals. As an example of t...

the purpose of this study was to develop a sensitive and fast microdialysis coupled with high-performance liquid chromatographic (hplc) method for determination of 5-hydroxymethyl-2-furaldehyde (5-hmf) in free-moving rats after i.g. administration of the aqueous extract of crud fructus corni and its processed products of jiuzheng pin (jzp). the concentration of 5-hmf in free-moving rats after i...