Cutaneous microdialysis is a new tool for direct sampling and real-time monitoring in dermatological research. However, its use in general laboratory practice poses several problems, one of which is determination of the relative recovery of the target material. Uric acid, one of the major antioxidants in the skin, was analysed using cutaneous microdialysis in 11 healthy subjects. Two methods fo...

This paper presents the use of tubing to store clinical microdialysis samples for delayed analysis with high temporal resolution, offering an alternative to traditional discrete offline microdialysis sampling. Samples stored in this way were found to be stable for up to 72 days at -80 °C. Examples of how this methodology can be applied to glucose and lactate measurement in a wide range of in vi...

introduction: sex differences are observed in the development of tolerance to antinociceptive effect of opioid drugs such as morphine, but the underlying mechanisms remain unclear. critical role of glutamate in the development and maintenance of opioid tolerance has been reported by many investigators. there are also evidences about interaction between gonadal hormones and neuromodulatory syste...

Root exudates are thought to promote nitrogen (N) availability via rhizosphere interactions, but empirical evidence is difficult obtain given the scale and temporary nature of these processes. Microdialysis has potential simulate root exudation patterns quantify effects on N simultaneously, this so far not been attempted. In a conceptual study, we used sucrose as simple C source investigate if ...

background: the present study evaluated the effects of treadmill running on extracellular basal levels of glutamate and gaba at dentate gyrus of streptozotocin-induced diabetic rats. methods: after 12 weeks of diabetes induction and exercise period, extracellular levels of glutamate and gaba were investigated. results: the results showed that glutamate levels were significantly decreased in dia...

Slice preparations are typically used to study the effects of pharmacological manipulations on the electrophysiological activity of mature neurons. However, the severing of afferent inputs is known to significantly change the natural membrane activity of the neuron. To study the effects of local pharmacological manipulations on neurons in the intact brain, we combined the methods of microdialys...

INTRODUCTION Lactate is a marker of hypoperfusion and may be used for risk assessment in critically ill patients. Although evidence suggests that repeated lactate measurements are of clinical interest, how and when lactate should be analyzed is controversial. Intravascular microdialysis provides a novel method for the continuous monitoring of lactate, which may be clinically beneficial in criti...

A direct, rapid and continuous in vivo monitoring of diffusible calcium in the blood of living rabbits has been developed using microdialysis sampling coupled on-line with flame atomic absorption spectrometry. Microdialysates perfused through implanted microdialysis probes were collected with a sample loop on an injection valve and directly introduced into the flame atomizer by a carrier soluti...

Microdialysis sampling of lipophilic molecules in human tissues is challenging because protein binding and adhesion to the membrane limit recovery. Hydroxypropyl-ß-cyclodextrin (HP-ß-CD) forms complexes with hydrophobic molecules thereby improving microdialysis recovery of lipophilic molecules in vitro and in rodents. We tested the approach in human subjects. First, we determined HP-ß-CD influe...

Högberg, N. 2013. Experimental and Clinical Necrotizing Enterocolitis. Acta Universitatis Upsaliensis. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 898. 47 pp. Uppsala. ISBN 978-91-554-8655-6. Necrotizing enterocolitis (NEC), a severe inflammatory disorder of the gastrointestinal tract with high morbidity and mortality, affects primarily preterm infants....