Microfluidic systems with splitting structures are excellent for increasing emulsion production. However, traditional two-dimensional (2D) lithographic systems require complex modification of the microchannel surfaces and achieve only 2D splitting of the droplets. Herein, we present new glass capillary microfluidic devices that perform three-dimensional (3D) splitting of droplets. These devices...

Cellular function and behavior are affected by the partial pressure of O2, or oxygen tension, in the microenvironment. The level of oxygenation is important, as it is a balance of oxygen availability and oxygen consumption that is necessary to maintain normoxia. Changes in oxygen tension, from above physiological oxygen tension (hyperoxia) to below physiological levels (hypoxia) or even complet...

Kinesin motor proteins carry out a range of transport functions in eukaryotic cells including long-distance transport in neurons and the movement of chromosomes during cell division. Because emerging microfluidic devices utilize channel geometries similar to cellular scales and because these devices generally require transport through these channels, there is significant interest in incorporati...

Ed Zwaan,1 Séverine Le Gac,2 Kinko Tsuji,3 and Claus-Dieter Ohl1 Faculty of Science and Technology, Physics of Fluids, University of Twente, Postbus 217, 7500 AE Enschede, The Netherlands. BIOS the Lab-on-a-Chip group, MESA+ Institute for Nanotechnology, University of Twente, Postbus 217, 7500 AE Enschede, The Netherlands. Shimadzu Europa GmbH, Albert-Hahn-Strasse 6-10, D-47269 Duisburg, German...

One in six couples of reproductive age worldwide are affected at least once by some form of infertility. In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are widely-available assisted reproductive technologies (ART). The identification and isolation of the most-motile sperm with DNA integrity are essential to IVF and ICSI, ultimately affecting treatment consequences and ...

This thesis is concerned with computational methods for fluid flows on the microscale, also known as microfluidics. This is motivated by current research in biological physics and miniaturization technology, where there is a need to understand complex flows involving microscale structures. Numerical simulations are an important tool for doing this. The first paper of the thesis presents a numer...

1. Image processing based position estimation for axial dimension 2. Kalman-filter based position estimation in the transverse plane 3. Details on microfluidic cell fabrication 4. SI Figure 1. Selected image frames obtained using sCMOS camera together with the corresponding binary transformation used to estimate particle axial position 5. SI Figure 2. Photograph of the transverse plane microflu...

Inkjet 3D printing is with success used for preparation microfluidics devices as a microfluidic chips for DNA separation. Utilized this technic help us to prepare a chip in shorter time and cheaper, but length of microchannel are limited by possibility of support material removing. One of the Solution for solve this limitations is divide a microfluidics chips for modules and printing them separ...

Microfluidics has been undergoing fast development in the past two decades due to its promising applications in biotechnology, medicine, and chemistry. Towards these applications, enhancing concentration sensitivity and detection resolution are indispensable to meet the detection limits because of the dilute sample concentrations, ultra-small sample volumes and short detection lengths in microf...

Liposomes are phospholipid-based self-assembled nanoparticles. Various components can be solubilized in the lipid bilayer, encapsulated aqueous core or attached to surface, making liposomes attractive platforms for multimodality functionalization. Here we describe theranostic delivering a magnetic resonance contrast agent (lipid derivative of gadopentetic acid) and hydrophobic photosensitizer (...