Abstract DNA barcoding allows the identification of an organism by comparing sequence selected regions (barcodes) with a previously compiled database, and it can be useful for taxonomic species in complex genera, such as Tamarix . Many this genus show convergent morphology, which leads to frequent errors their identification. Highly variable genetic markers, microsatellites or short repeats (SS...

Microsatellites are the most promising co-dominant markers, widely distributed throughout the genome. Identification of these repeating genomic subsets is a tedious and iterative process making computational approaches highly useful for solving this biological problem. Here 38,083 microsatellites were localized in palm sequences. A total of 2, 97,023 sequences retrieved from public domains were...

The advent of DNA technology over the recent years has led to the development of molecular markers that are highly precise, convenient and cost effective for detection of polymorphism among individuals and also for individual identification. These molecular marker techniques viz., Restriction fragment length polymorphism, Random amplified polymorphic DNA, Amplified fragment length polymorphism,...

Single-nucleotide polymorphisms (SNPs) are a class of attractive genetic markers for population genetic studies and for identifying genetic variations underlying complex traits. However, the usefulness and efficiency of SNPs in comparison to microsatellites in different scientific contexts, e.g., population structure inference or association analysis, still must be systematically evaluated thro...

To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed the gene content of representative members of the subfamily, and determined whether individual genes present in each genome were intact, truncated, or fragmented. When nonintact genes were identified, the early stop mutations (ESMs) leading to gene truncation or fragmentation were analyzed. Of all th...

Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (SNPs) make them ano...

Recent technical advances allow straightforward access to genetic information directly drawn from DNA. The present article highlights the suitability of high variation molecular genetic markers, such as microsatellites, for studies relevant to amphibian conservation. Molecular markers appear particularly useful for i) measuring local gene flow and migration, ii) assigning individuals to their m...

The European Collaborative Interspecific Backcross (EUCIB) resource was constructed for the purposes of high-resolution genetic mapping of the mouse genome (). The large Mus spretus/C57BL/6 backcross of 982 progeny has a genetic resolution of 0.3 cM at the 95% confidence level ( approximately 500 kb in the mouse genome). We have used the EUCIB mapping resource to develop a genome-wide high-reso...

A procedure for selection of wheat genomic fragments containing various microsatellites is described. The procedure is based on the hybridization of single-stranded DNA fragments either to filter bound microsatellite DNA or to biotinylated oligonucleotides in solution. Hybridized strands are isolated, amplified and cloned. Results presented show that enrichment for (CA)n/(TG)n microsatellites d...

The SilkSatDb (silkmoth microsatellite database) (http://www.cdfd.org.in/silksatdb) is a relational database of microsatellites extracted from the available expressed sequence tags and whole genome shotgun sequences of the silkmoth, Bombyx mori. The database has been rendered with a simple and robust web-based search facility, developed using PHP. The SilkSatDb also stores information on primer...