Microsatellites are a major component of the human genome, and their evolution has been much studied. However, the evolution of microsatellite flanking sequences has received less attention, with reports of both high and low mutation rates and of a tendency for microsatellites to cluster. From the human genome we generated a database of many thousands of (AC)(n) flanking sequences within which ...

During the construction of a primary linkage map of the sheep genome, a total of 46,225 parent-offspring transfer of microsatellite alleles were examined through the genotyping of the AgResearch International Mapping Flock. Any observations of mutations were thoroughly rechecked, including examination of new DNA samples, to yield five bona fide mutations from the study. This gave us an observed...

Interspersed and tandem repeat sequences comprise the bulk of mammalian genomes. Interspersed repeats result from successive replication by transposable elements, such as Alu and long interspersed element type 1 (L1). Microsatellites are tandem repeats of 1-6 base pairs, among which poly(A) microsatellites are the most abundant in the human genome. The rise and fall of a microsatellite has been...

tempranillo is one of the most widely cultivated grapevine varieties in spain. after several years of clone selection, some highly recommended old clones have been identified in terms of both their qualitative and production characteristics. this study was designed to discriminate among 28 ancient clones of the cultivar tempranillo (vitis vinifera). dna samples from clones were analysed using t...

two breeds of chickens were used to develop reference family and detect qtls affecting egg quality traits: cocks of a native polish breed green-legged partidgenous (glp) and hens of rhode island red (rir). during the first 100 days of the laying period, the glp hens lay about 40 eggs weighting on average 50 g, whilst the rir flock laid 83 eggs with a mean weight of 60 g. qtl analysis of egg qua...

While the faithful transmission of genetic information requires a fidelity and stability of DNA that is involved in translation into proteins, it has become evident that a large part of noncoding DNA is organized in repeated sequences, which often exhibit a pronounced instability and dynamics. This applies both to longer repeated sequences, minisatellites (about 10-100 base pairs), and microsat...