The irregularity of growing and shortening patterns observed experimentally in microtubules their ensembles reflects a dynamical system that fluctuates stochastically between assembly disassembly phases. time series microtubule lengths have been extensively analyzed to shed light on structural properties microtubules. Here, for the first time, Multifractal Detrended Fluctuation analysis (MFDFA)...

In this paper, we use the modified exp−ψθ-function method to observe some of solitary wave solutions for microtubules (MTs). By treating issues as nonlinear model partial differential equations describing microtubules, were able solve problem. We then found specific evolution equation (NLEE) covering various parameters that are particularly significant in biophysics and nanobiosciences. additio...

Cell stiffening induced by anticancer microtubule targeting drugs (MTDs) such as vinflunine (VFL), colchicine (COL), and docetaxel (DTX) can be driven crosstalk between actin filaments microtubules.

Abstract The cytoskeleton determines cell mechanics and lies at the heart of important cellular functions. Growing evidence suggests that manifold tasks rely on interactions between its filamentous components—actin filaments, intermediate microtubules. However, nature these their impact cytoskeletal dynamics are largely unknown. Here, we show in a reconstituted vitro system vimentin filaments s...

Disassembling microtubules provide the driving forces behind chromosomal movement in dividing cells. This motion can occur independently of any conventional motor activity, and instead relies on tenacious attachment kinetochores to their dynamic microtubule substrates. During disassembly, thousands tubulin subunits are lost from tip per second even under tension, must maintain crumbling substra...

During metaphase, chromosome position at the mitotic spindle equator is regulated by forces exerted kinetochore microtubules and polar ejection forces. However, role of arising from mechanical coupling sister fibers with bridging in alignment unknown. Here we develop an optogenetic approach for acute removal microtubule crosslinker PRC1 to disassemble show that they promote alignment. Tracking ...

Transport of intracellular cargos along microtubules is essential for the function and survival eukaryotic cells. While we know much about motors which perform this transport, significantly less known how transport controlled directed. One step process remains little understood binding to microtubules. In poster, explore what we’ve learned through several recent studies. We look at in different...

Microtubules that assemble the mitotic spindle are generated by centrosomal nucleation, chromatin-mediated and nucleation from surface of other microtubules mediated augmin complex. Impairment in apical progenitors developing mouse brain induces p53-dependent apoptosis causes non-lethal microcephaly. Whether disruption non-centrosomal has similar effects is unclear. Here, we show, using embryos...

Abstract The cytoskeleton is a major focus of physical studies to understand organization inside cells given its primary role in cell motility, division, and mechanics. Recently, protein condensation has been shown be another intracellular organizational strategy. Here, we report that the microtubule crosslinking proteins, MAP65-1 PRC1, can form phase separated condensates at physiological salt...