PU.1, an Ets family transcription factor, was previously demonstrated expressed in 3T3-L1 preadipocytes and had an negative effect on adiopogenesis. However, the underlying mechanism remains elusive. Here, miR-191 was identified as an inhibitor of adipocyte differentiation through targeting the 3' untranslated regions of C/EBPβ, the initial factor in the C/EBPα/β-PPARγ terminal pathway of adipo...

S oon after their discovery in 1993, it became clear that microRNAs (miRs) regulate virtually all cellular processes. 1 Since then, many miRs have been described to coordinate gene expression programs in the context of cardiovascular disease, including diabetes mellitus. 2,3 MiRs usually perform their duties intracellularly, but interest in extracellular miRs as biomarkers or intercellular mess...

Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and development...

The molecular mechanisms of hypoxia induced breast cell migration remain incompletely understood. Our results show that hypoxia through hypoxia-inducible factor (HIF) brings about a time-dependent increase in the level of an oncogenic microRNA, miR-191 in various breast cancer cell lines. miR-191 enhances breast cancer aggressiveness by promoting cell proliferation, migration and survival under...

As a family of post-transcriptional regulator of gene expression, the microRNAs (miRNAs) control a wide array of biological processes including cell differentiation, proliferation and apoptosis, and the dysregulation of miRNAs is a hallmark of cancer. Here, we found that the microRNA-191 (miR-191) was at a high-expression level in human gastric adenocarcinoma cell line MGC803 and human gastric ...

Themolecular mechanisms of hypoxia induced breast cell migration remain incompletely understood. Our results show that hypoxia through hypoxia-inducible factor (HIF) brings about a time-dependent increase in the level of an oncogenicmicroRNA,miR-191 in various breast cancer cell lines.miR-191 enhances breast cancer aggressiveness by promoting cell proliferation, migration and survival under hyp...

We aimed at assessing the stability of candidate reference genes in urine sediments of men subjected to digital rectal examination for suspected prostate cancer (PCa). Two microRNAs (miR-191 and miR-25) and 1 small nucleolar RNA (SNORD48) were assayed in 35 post-DRE urine sediments of men with PCa and in 26 subjects with histologically confirmed benign prostatic hyperplasia (BPH). The stability...

Downlo atocellular carcinoma (HCC) is generally a fatal disease due to a paucity of effective treatment options. entification of oncogenic microRNAs that exert pleiotropic effects in HCC cells may offer new theratargets. In this study, we have identified the human microRNA miR-191 as a potential target for HCC y. Inhibition of miR-191 decreased cell proliferation and induced apoptosis in vitro ...

Hepatocellular carcinoma (HCC) is generally a fatal disease due to a paucity of effective treatment options. The identification of oncogenic microRNAs that exert pleiotropic effects in HCC cells may offer new therapeutic targets. In this study, we have identified the human microRNA miR-191 as a potential target for HCC therapy. Inhibition of miR-191 decreased cell proliferation and induced apop...

Serum microRNAs (miRNAs) have become a highlighted research hotspot, especially for their great potential as a novel promising non-invasive biomarker in cancer diagnosis. The most frequently used approach for serum miRNAs detection is quantitative real time polymerase chain reaction (qPCR). In order to obtain reliable qPCR data of miRNAs expression, the use of reference genes as endogenous cont...