A functional rs4245739 A>C single nucleotide polymorphism (SNP) locating in the MDM43'-untranslated (3'-UTR) region creates a miR-191-5p or miR-887-3p targeting sites. This change results in decreased expression of oncogene MDM4. Therefore, we examined the association between this SNP and small cell lung cancer (SCLC) risk as well as its regulatory function in SCLC cells. Genotypes were determi...

BACKGROUND Recent studies have revealed the role of microRNAs (miRNAs) in a variety of biological and pathological processes, including acute myocardial infarction (AMI). We hypothesized that ST-segment elevation myocardial infarction (STEMI) may be associated with an alteration of miRNAs and that circulating miRNAs may be used as diagnostic markers for STEMI. METHODS Expression levels of 270...

Since proprotein convertase subtilisin kexin 9 (PCSK9) discovery, a gene involved in LDL metabolism regulation and cardiovascular diseases (CVD), many therapeutic strategies have been introduced for direct targeting of PCSK9. The main goal of these strategies has been to reduce PCSK9 protein level either by application of antibodies or inhibition of its production. In this study, we have tried ...

objective: in spite of accumulating information about pathological aspects of sulfur mustard (sm), the precise mechanism responsible for its effects is not well understood. circulating micrornas (mirnas) are promising biomarkers for disease diagnosis and prognosis. accurate normalization using appropriate reference genes, is a critical step in mirna expression studies. in this study, we aimed t...

Specific microRNAs have emerged as key players in disease biology by playing crucial role in disease development and progression. This review draws attention to one such microRNA, miR-191 that has been recently reported to be abnormally expressed in several cancers (>20) and various other diseases like diabetes-type 2, Crohn' s, pulmonary hypertension, and Alzheimer' s. It regulates important c...

Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal human cancers chronic hepatitis B virus (HBV) infection accounts for up to 50% total HCC cases worldwide. Although resection surgery has been available as a curative therapy HCC, high rate recurrence remains big threat patient survival. Therefore, discovery valuable biomarkers selecting patients at higher risk key goal allow...

RATIONALE MicroRNA (miRNA) biomarkers are attracting considerable interest. Effects of medication, however, have not been investigated thus far. OBJECTIVE To analyze changes in plasma miRNAs in response to antiplatelet therapy. METHODS AND RESULTS Profiling for 377 miRNAs was performed in platelets, platelet microparticles, platelet-rich plasma, platelet-poor plasma, and serum. Platelet-ric...

Of the over 200 identified mammalian microRNAs (miRNAs), only a few have known biological activity. To gain a better understanding of the role that miRNAs play in specific cellular pathways, we utilized antisense molecules to inhibit miRNA activity. We used miRNA inhibitors targeting miR-23, 21, 15a, 16 and 19a to test efficacy of antisense molecules in reducing miRNA activity on reporter genes...