We previously reported an impaired ability to regulate hepatic mitochondrial state 4 respiration rate in response to sequential additions of adenosine diphosphate in pulmonary hypertension syndrome (PHS). As proton conductance is a major contributor to State 4 respiration, the major goal of this study was to investigate the nature of proton conductance in hepatic mitochondria isolated from broi...

Using dual excitation and fixed emission fluorescence microscopy, we were able to measure changes in cytoplasmic free Ca(2+) concentration ([Ca(2+)](i)) and mitochondrial membrane potential simultaneously in the pancreatic beta-cell. The beta-cells were exposed to a combination of the Ca(2+) indicator fura-2/AM and the indicator of mitochondrial membrane potential, rhodamine 123 (Rh123). Using ...

Delcamp, T. J., C. Dales, L. Ralenkotter, P. S. Cole, and R. W. Hadley. Intramitochondrial [Ca21] and membrane potential in ventricular myocytes exposed to anoxia-reoxygenation. Am. J. Physiol. 275 (Heart Circ. Physiol. 44): H484– H494, 1998.—The aim of this study was to investigate the role of mitochondrial ionic homeostasis in promoting reoxygenation-induced hypercontracture in cardiac muscle...