Sambhar Lake (Salt of Earth), the largest inland saline and alkaline lake of India is situated in the state of Rajasthan, and is referred in the epic Mahabharata. When full, it covers an area of 230 sq km. It lays some 60 km west of Jaipur, adjacent to the Salt Lake City. Flocks of flamingoes are attracted by the delicious spirulina algae that flourish when the water reaches a medium degree of ...

BACKGROUND Extensive use of phenolic compounds in industry has resulted in the generation of saline wastewaters that produce significant environmental contamination; however, little information is available on the degradation of phenolic compounds in saline conditions. Halomonas organivorans G-16.1 (CECT 5995(T)) is a moderately halophilic bacterium that we isolated in a previous work from sali...

In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional molecular methods. 16S rRNA gene sequence analyses showed that belonged to Planococcus dechangensis species. It gave positive result in Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative colony pigmentation yellow-orange. resul...

BACKGROUND Microbial culturomics represents an ongoing revolution in the characterization of environmental and human microbiome. METHODS By using three media containing high salt concentration (100, 150, and 200 g/L), the halophilic microbial culturome of a commercial table salt was determined. RESULTS Eighteen species belonging to the Terrabacteria group were isolated including eight moder...

background: today a large number of bacterial amylases are available commercially in industry. the goal of the present study was purification and biochemical characterization of an extracellular thermostable alkaline α-amylase from the novel moderately halophilic, bacillus persicus from the aran-bidgol, iran.   methods : purification of enzyme, was carried out by acetone precipitation, ultrafil...

Protein-solvent interactions govern the behaviors of proteins isolated from extreme halophiles. In this work, we compared the solvent envelopes of two orthologous tetrameric malate dehydrogenases (MalDHs) from halophilic and non-halophilic bacteria. The crystal structure of the MalDH from the non-halophilic bacterium Chloroflexus aurantiacus (Ca MalDH) solved, de novo, at 1.7 Å resolution exhib...

It has been known that halophilic bacteria often show natural resistance to antibiotics, dyes, and toxic metal ions, but the mechanism and regulation of this resistance have remained unexplained. We have addressed this question by identifying the gene responsible for multidrug resistance. A spontaneous ofloxacin-resistant mutant derived from the moderately halophilic bacterium Chromohalobacter ...

The role of choline in osmoprotection in the moderate halophile Halomonas elongata has been examined. Transport and conversion of choline to betaine began immediately after addition of choline to the growth medium. Intracellular accumulation of betaine synthesized from choline was salt dependent up to 2.5 M NaCl. Oxidation of choline was enhanced at 2.0 M NaCl in the presence or absence of exte...

Two moderately halophilic sulfate-reducing bacteria were isolated from an African oil pipeline and designated strains SEBR 3640 and SEBR 2840T (T = type strain). Both of these strains possess traits that define the genus Desulfovibrio. The cells of both isolates were motile curved rods that had a single polar flagellum and contained desulfoviridin, and both isolates utilized lactate, pyruvate, ...

Halophilic microorganisms are source of potential hydrolytic enzymes to be used in industrial and/or biotechnological processes. In the present study, we have investigated the ability of the moderately halophilic bacterium Halobacillus blutaparonensis (strain M9), a novel species described by our group, to release proteolytic enzymes. This bacterial strain abundantly proliferated in Luria-Berta...