in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

B1-xLaxFeO3 (x = 0.0, 0.025) crystallites were prepared by chemical solution route. XRD analysis confirmed the phase formation of the materials. Thermal characterization was done by DSC-TGA to confirm crystallization and thermal behavior for phase development. Morphological studies by FESEM showed the agglomerated nature of the ferrite...

B1-xLaxFeO3 (x = 0.0, 0.025) crystallites were prepared by chemical solution route. XRD analysis confirmed the phase formation of the materials. Thermal characterization was done by DSC-TGA to confirm crystallization and thermal behavior for phase development. Morphological studies by FESEM showed the agglomerated nature of the ferrite...

in this study, molecular characterization of echinococcus granulosus sample obtained from water buffalo originating from southwest of iran was performed using comparative sequence analysis of cox1 mitochondrial gene. dna was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in khuzestan province. molecular and phylogenetic analyses we...

millions of diarrheal disease is made by enterotoxigenic e. coli (etec) each year, specifically in developing countries. in the pathogenesis of etec infections, the first phase is sticking of the bacterium to the minute intestinal epithelium, as a result of colonization factors (cfs) mediation and subsequently generate enterotoxins. these cfs in accordance with their structure are diverged into...

background: feline hemotropic mycoplasmas are parasites of erythrocytes and include three species, mycoplasma haemofelis, candidatus mycoplasma haemominutum and candidatus mycoplasma turicensis. diagnosis of the infection with these microorganisms can be carried out using conventional assays such as blood cytology. however, these assays have a low accuracy and a high rate of false-positive resu...

background: coccidiosis is a serious protozoal disease of poultry. the iden-tification of eimeria species has important implications for diagnosis and con-trol as well as for epidemiology. the molecular characterization of eimeria spe-cies infecting egyptian baladi chickens was investigated. methods: eimeria species oocysts were harvested from intestines of naturally infected egyptian baldi chi...

The narrow genetic base of soybean makes cultivar characterization based on morphological descriptors difficult; this characterization is mainly done for registration and protection. Correct characterization of cultivars could be achieved through molecular markers, since the frequencies of each allele in the population are known. Consequently, we developed a molecular characterization method an...

In this paper, we present characterizations of pairs of graphs whose join graphs are 2-minimally nonouterplanar. In addition, we present a characterization of pairs of graphs whose join graphs are 2-minimally nonouterplanar in terms of forbidden subgraphs.

For the family of graphs with xed-size vertex cover k, we present all of the forbidden minors (obstructions), for k up to ve. We derive some results, including a practical nite-state recognition algorithm, needed to compute these obstructions.