Background Patients with systemic lupus erythematosus (SLE) suffer from a high incidence of premature ovarian failure, which might be due to cyclophosphamide gonadal toxicity, immune abnormalities, or other reasons. This study aimed investigate whether the transplantation human umbilical cord mesenchymal stem cells (HUC-MSCs) can improve reserve function in mice. Methods We used MRL/lpr mice ob...

BACKGROUND Fetal bovine serum (FBS) is widely used in cell culture laboratories, risk of zoonotic infections and allergic side effects create obstacles for its use in clinical trials. Therefore, an alternative supplement with proper inherent growth-promoting activities is demanded. OBJECTIVE To find FBS substitute, we tested human umbilical cord blood serum (hUCS) for proliferation of human u...

 ABSTRACT Background: Cholesterol cleft granulomas with clusters of giant cells were noted to be a common feature of non-specific interstitial pneumonia (NSIP). Objective: This study aimed to define the cell populations involved in the granulomas. Methods: The granulomas of 16 patients with cryptogenic fibrosing alveolitis (five cases with the histological features ofNSIP, five with those ofUIP...

background , despite numerous studies over a decade, it still remains controversial about the biological effects of rf emf emitted by mobile phone telephony. objective , here we investigated the effect of 900 mhz gsm on the induction of oxidative stress and the level of intracellular reactive oxygen species (ros) in human mononuclear cells, monocytes and lymphocytes as defence system cells. met...

introduction: while human endothelial progenitor cells (epcs) have been a subject of somehow extensive investigation, epcs from adult mouse hematopoietic system were poorly studied. present investigation is focused on fvb mouse endothelial progenitor cells in terms of their isolation, purification, and expansion. materials and methods: mononuclear cells collected from murine peripheral blood we...

results of 45 patients, 4 (8.9% [95% ci: 4.4-15.6]) had detectable genomic hcv-rna in their pbmc specimens. hcv genotypes were determined in the pbmcs of these subjects showed that 2 (50.0%) subjects with occult hcv infection had hcv subtype 3a, and 2 (50.0%) had hcv subtype 1b. conclusions this study found that 8.9 % of the iranian candidates for liver transplant with cryptogenic cirrhosis had...

abstract background: cholesterol cleft granulomas with clusters of giant cells were noted to be a common feature of non-specific interstitial pneumonia (nsip). objective: this study aimed to define the cell populations involved in the granulomas. methods: the granulomas of 16 patients with cryptogenic fibrosing alveolitis (five cases with the histological features ofnsip, five with those ofuip ...

Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have recently been recognized as a potential source for cell-based therapy in various preclinical animal models, such as Parkinson's disease, cerebral ischemia, spinal cord injury, and liver failure; however, the precise cellular and molecular mechanisms underlying the beneficial outcomes remain under investigation. There is a growi...

Human urinary disorders are generally studied in rodent models due to limitations of functional in vitro culture models of primary human urothelial cells (HUCs). Current HUC culture models are often derived from immortalized cancer cell lines, which likely have functional characteristics differ from healthy human urothelium. Here, we described a simple explant culture technique to generate HUCs...